Factor VIII R Antigen antibody

Antigen: Factor VIII R Antigen

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Immunohistochemistry (IHC)

Catalog no.: ABIN99043

Additional Information

Immunogen: Factor VIII related antigen isolated from human plasma.

Format: Ig-fraction

Specificity: This antibody reacts with human factor VIII related antigen. It stains endothelial cells and megakaryocytes in various human tissues. This antibody does not cross-react with any other cell types.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:50-1:100 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions should be determined by the user. Although not required, proteolytic treatment of formalin fixed paraffin embedded tissue sections may enhance the immunostaining in overfixed tissues. Positive Control: Tonsil. Cellular localization: Cytoplasmic

Concentration: 100ug/ml approximately

Buffer: Purified immunoglobulin fraction of rabbit antiserum against human factor VIII related antigen containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Research Area: Coagulation

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Images

Formalin fixed paraffin embedded human tonsil stained with Factor VIII antibody

Publications

Publications: Bukh, Ingerslev, Stenbjerg et al.: "The multimeric structure of plasma F VIII:RAg studied by electroelution and immunoperoxidase detection." in: Thrombosis research, Vol. 43, Issue 5, pp. 579-84, 1986 (PubMed).

Sehested, Hou-Jensen: "Factor VII related antigen as an endothelial cell marker in benign and malignant diseases." in: Virchows Archiv. A, Pathological anatomy and histology, Vol. 391, Issue 2, pp. 217-25, 1981 (PubMed).

Cejka: "Enzyme immunoassay for factor VIII-related antigen." in: Clinical chemistry, Vol. 28, Issue 6, pp. 1356-8, 1982 (PubMed).