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Factor VIII Related Antigen (FVIII-Ag) (Factor 8) antibody

Antigen

Factor VIII Related Antigen (FVIII-Ag)

Synonyms F8, Cf8, Cf-8, FVIII
Epitope

Factor 8

Clonality Polyclonal
Host

Rabbit

Reactivity

Human

Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
3 references available
Catalog no. ABIN99044
Quantity 6ml  (1 to 4 ug/ml)
Price 111.00 $   Plus shipping costs $35.00
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Additional Information

Alternative name Factor VIII (Factor 8) related antigen
Immunogen Factor VIII related antigen isolated from human plasma.
Format Ig-fraction, Liquid (prediluted)
Specificity This antibody reacts with human factor VIII related antigen. It stains endothelial cells and megakaryocytes in various human tissues. This antibody does not cross-react with any other cell types.

Application Details

Application Notes Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions should be determined by the user. Suitable for formalin/paraffin tissues. Although not required, proteolytic treatment may enhance the immunostaining in overfixed tissues. Positive Control: Tonsil. Cellular localization: Cytoplasmic
Concentration 1 to 4 ug/ml
Buffer This antibody has been pretitered and quality controlled to work on formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections. No further titration is required.
Storage Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.
Research Area Coagulation
Restrictions For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Publications

Publications Bukh, Ingerslev, Stenbjerg et al.: "The multimeric structure of plasma F VIII:RAg studied by electroelution and immunoperoxidase detection." in: Thrombosis research, Vol. 43, Issue 5, pp. 579-84, 1986 (PubMed).

Sehested, Hou-Jensen: "Factor VII related antigen as an endothelial cell marker in benign and malignant diseases." in: Virchows Archiv. A, Pathological anatomy and histology, Vol. 391, Issue 2, pp. 217-25, 1981 (PubMed).

Cejka: "Enzyme immunoassay for factor VIII-related antigen." in: Clinical chemistry, Vol. 28, Issue 6, pp. 1356-8, 1982 (PubMed).