Coagulation Factor XIII, A1 Polypeptide (F13A1) (Factor 13a) antibody

Antigen: Coagulation Factor XIII, A1 Polypeptide (F13A1)

Synonyms: F13a, F13A1, wu:fo83d04, zgc:153334, F13A, AI462306, 1200014I03Rik

Epitope: Factor 13a

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN99068

Additional Information

Alternative name: Factor XIIIa (Factor 13a)

Immunogen: Recombinant protein corresponding to A-subunit of coagulation Factor XIII a.

Format: Ig-fraction, Purified

Specificity: This antibody reacts with a 160 kD (unreacted dimer) and 80 kD (reduced monomer) protein. Factor XIII is a beta-globulin found in plasma and is composed of two subunits. Factor XIII-A is the catalytic subunit and is a dimmer of M.W. 160 kD. Factor XIII is a dermal dendrocyte marker and shows variable reaction with these types of tumors. Factor XIII can be used for histiocytic phenotyping and has been reported to mark capillary hemangiomas and tumors of the central nervous system.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Placenta. Cellular localization: Cytoplasmic, nuclear

Concentration: 100ug/ml approximately

Buffer: Purified immunoglobulin fraction of rabbit antiserum against Factor XIII a containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Research Area: Coagulation

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union