Fetoprotein antibody

Antigen: Fetoprotein

Clonality: Monoclonal (C3)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (IHC)

Catalog no.: ABIN99162

Additional Information

Immunogen: BALB/C mice were injected with purified human alpha- fetoprotein.

Format: Ig-fraction

Isotype: IgG2a  (Matching secondary antibodies)

Clone: C3

Specificity: Alpha-Fetoprotein (AFP) is a single chain glycoprotein of 70 kD that is normally expressed in the fetal liver, gastrointestinal tract and yolk sac. This antibody recognizes an epitope of alpha- Fetoprotein present in human and several other species. It cross reacts with human, dog and pig but not with bovine, cat, rat and mouse.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Suitable for formalin/paraffin tissues. Positive Control: Fetal liver. Cellular localization: Cytoplasmic

Concentration: 100ug/ml approximately

Buffer: This antibody is supplied as diluted ascites containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Publications

Publications: Chan, Kelsten, Rock et al.: "Evaluation of a monoclonal immunoenzymometric assay for alpha-fetoprotein." in: Clinical chemistry, Vol. 32, Issue 7, pp. 1318-22, 1986 (PubMed).

Abelev: "Alpha-fetoprotein in ontogenesis and its association with malignant tumors." in: Advances in cancer research, Vol. 14, pp. 295-358, 1972 (PubMed).