Fibronectin 1 (FN1) antibody

Antigen: Fibronectin 1 (FN1)

Synonyms: FN, CIG, FNZ, MSF, ED-B, FINC, GFND, LETS, GFND2, DKFZp686H0342, DKFZp686I1370, DKFZp686F10164, DKFZp686O13149, Fn, Fn-1, MGC117493, E330027I09, fn-1, FIBNEC, FN1, cig, msf, finc, lets, MGC97508, fibronectin, fn2, fb80d10, wu:fb80d10

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Immunohistochemistry (IHC)

Catalog no.: ABIN99276

Additional Information

Alternative name: Fibronectin

Immunogen: Fibronectin isolated from a pool of normal human plasma.

Format: Ig-fraction

Specificity: This antibody reacts with human fibronectin in liver, tonsil, skin and kidney. Traces of contaminating antibodies have been removed by solid-phase absorption. Only the fibronectin arch was observed in crossed immunoelectrophoresis.

Synonyms: FN, CIG, FNZ, MSF, ED-B, FINC, GFND, LETS, GFND2, DKFZp686H0342, DKFZp686I1370, DKFZp686F10164, DKFZp686O13149, Fn, Fn-1, MGC117493, E330027I09, fn-1, FIBNEC, FN1, cig, msf, finc, lets, MGC97508, fibronectin, fn2, fb80d10, wu:fb80d10

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:50-1:250 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions should be determined by the user. Suitable for formalin/paraffin tissues. Positive Control: Kidney. Cellular localization: Basement membrane, connective tissue

Concentration: 100ug/ml approximately

Buffer: Purified immunoglobulin fraction of rabbit antiserum against human fibronectin containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Research Area: Extracellular Matrix

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Images

Formalin fixed paraffin embedded human kidney stained with Fibronectin antibody

Publications

Publications: dArdenne, Kirkpatrick, Wells et al.: "Laminin and fibronectin in adenoid cystic carcinoma." in: Journal of clinical pathology, Vol. 39, Issue 2, pp. 138-44, 1986 (PubMed).

Kirkpatrick, dArdenne: "Effects of fixation and enzymatic digestion on the immunohistochemical demonstration of laminin and fibronectin in paraffin embedded tissue." in: Journal of clinical pathology, Vol. 37, Issue 6, pp. 639-44, 1984 (PubMed).

Kataoka, Sakanaka, Semma et al.: "PGE2-receptor subtype EP4-dependent adherence of mastocytoma P-815 cells to matrix components in subcutaneous tissues overlaying inside surface of air pouch cavity in CDF1 mouse." in: Inflammation research : official journal of the European Histamine Research Society ... [et al.], Vol. 57, Issue 8, pp. 362-6, 2008 (PubMed).