GABA antibody

Antigen: GABA

Clonality: Polyclonal

Host: Rabbit

Application: Immunocytochemistry (ICC)

Catalog no.: ABIN99458

Additional Information

Immunogen: Synthetic GABA conjugated to protein carrier (Pc)

Format: Lyophilized

Isotype: IgG  (Matching secondary antibodies)

Specificity: Conjugated -aminobutyric acid (GABA) . Using a conjugate GABA-(Pc), antibody specificity was performed with an ELISA test by competition experiments with the following compounds: Compounds Cross-reactivity ratio (a) GABA-G-(Pc) 1 -Alanine-G-(Pc) 1/10,000 D-Glutamate-G-(Pc) 1/> 10,000 Glycine-G-(Pc) 1/20,000 D-Aspartate-G-(Pc) 1/> 50,000 (a): GABA-G-(Pc) concentration/Conjugated aminoacid concentration at half displacement G = Glutaraldehyde

Application Details

Application Notes: Recommended dilutions for Immunocytochemistry (1/1,000-5,000) Recommended dilutions for Western Blot (1/1,000-1/2,000)

Purity: Antiserum previously preabsobed on protein carriers, and purified.

Storage: Lyophilized antibodies are stable at least 2 years. After reconstitution with 50µl of distilled water and 50µl of glycerol, the aliquot can be repeated freezed (up to five times).

Research Area: Neurotransmitters, Ion Channels

Restrictions: For Research Use only

Publications

Publications: Gamrani, Onteniente, Seguela et al.: "Gamma-aminobutyric acid-immunoreactivity in the rat hippocampus. A light and electron microscopic study with anti-GABA antibodies." in: Brain research, Vol. 364, Issue 1, pp. 30-8, 1986 (PubMed).

Geffard, DeFeudis, Varga et al.: "Decreases in the binding of [3H]GABA and [3H]muscimol to a particulate fraction of rat brain produced by human sera; some speculations." in: General pharmacology, Vol. 11, Issue 5, pp. 425-8, 1980 (PubMed).