Galectin, Galactoside-Binding, Soluble, 3 (LGALS3) antibody

Antigen: Galectin, Galactoside-Binding, Soluble, 3 (LGALS3)

Synonyms: galectin-3, LGALS3, Galectin-3, MGC148757, leg3

Clonality: Monoclonal (9C4)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN99547

Additional Information

Alternative name: Galectin-3

Immunogen: BALB/C mice were immunized with prokaryotic recombinant fusion protein corresponding to the full-length galectin-3 molecule.

Format: Supernatant

Isotype: IgG1  (Matching secondary antibodies)

Clone: 9C4

Specificity: Galactin-3 is a member of the beta-galactosidase binding lectin family. Cell surface galectin-3 may be involved in homotypical cell adhesion and is down

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:50-1:75 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 60 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Prostate. Cellular localization: Cytoplasmic

Concentration: 100ug/ml approximately

Buffer: This antibody is supplied as tissue culture supernatant containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Research Area: Neurology

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Publications

Publications: Castronovo, Van Den Bruule, Jackers et al.: "Decreased expression of galectin-3 is associated with progression of human breast cancer." in: The Journal of pathology, Vol. 179, Issue 1, pp. 43-8, 1996 (PubMed).

Kasper, Hughes: "Immunocytochemical evidence for a modulation of galectin 3 (Mac-2), a carbohydrate binding protein, in pulmonary fibrosis." in: The Journal of pathology, Vol. 179, Issue 3, pp. 309-16, 1996 (PubMed).

Gasbarri, Martegani, Del Prete et al.: "Galectin-3 and CD44v6 isoforms in the preoperative evaluation of thyroid nodules." in: Journal of clinical oncology : official journal of the American Society of Clinical Oncology, Vol. 17, Issue 11, pp. 3494-502, 2000 (PubMed).