Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2) antibody

Antigen: Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2)

Synonyms: GAD65, MGC161605, MGC161607, Gad-2, GAD(65), 6330404F12Rik, gad65, zgc:112198, GAD2, F12P19.12, F12P19_12, GLUTAMATE DECARBOXYLASE 2

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Rat (Rattus)

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN99797

Additional Information

Alternative name: Glutamate Decarboxylase 65 (GAD65)

Immunogen: A synthetic peptide derived from the C-terminus region of the human GAD65 protein.

Format: Ig-fraction, Purified

Specificity: This antibody reacts with a 65 kD protein, known as GAD (Glutamate Decarboxylase). Two forms of GAD exist in adults, GAD65 and GAD67, which are encoded by different genes. GAD65 and GAD67 are expressed in most GABA containing neurons. GAD65 appears to be targeted to the membrane and nerve ending, where as GAD67 is more widely distributed throughout the cell. This antibody cross reacts with rat.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Pancreas. Cellular localization: Cytoplasmic

Concentration: 100ug/ml approximately

Buffer: Purified immunoglobulin fraction of rabbit antiserum against human GAD65 containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Research Area: Enzymes, Metabolism

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Publications

Publications: Karlsen, Hagopian, Grubin et al.: "Cloning and primary structure of a human islet isoform of glutamic acid decarboxylase from chromosome 10." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 88, Issue 19, pp. 8337-41, 1991 (PubMed).

Bu, Tobin: "The exon-intron organization of the genes (GAD1 and GAD2) encoding two human glutamate decarboxylases (GAD67 and GAD65) suggests that they derive from a common ancestral GAD." in: Genomics, Vol. 21, Issue 1, pp. 222-8, 1994 (PubMed).