14-3-3 (Rat) antibody

Overview

Antigen	14.03.2003
Immunogen	Synthetic peptide, corresponding to amino acids 208-244 of Rat 14-3-3.
Reactivity	Rat
Antibody Type	Monoclonal
Format	Liquid
Isotype	IgG2a »Matching secondary antibodies
Description	Members of the 14-3-3 family of proteins are highly conserved proteins, localized in neurons, and are axonally transported to the nerve terminals. They are also present, at lower levels, in various other eukaryotic tissues. 14-3-3 proteins appear to play important roles in a variety of signal transduction pathways, including those involved in cell cycle regulation and cell survival. Because 14-3-3 proteins bind to specific phosphoserine-containing sequences they are likely to have an important role in signaling pathways mediated by serine/threonine protein kinases. Evidence indicates 14-3-3 is required for Raf-1 kinase activity and phosphorylation amoung many other functions.
Clone	2Q248
Host	Mouse
Specificity	Detection varies from tissue to tissue (Brain, Retina, Testis, Pineal, Kidney, Spleen, Adrenal, Lung, Heart, Liver, Ovary, Thyroid) and during development. This is a pan specific 14-3-3 antibody.

Application	Western Blotting (WB)
Application Notes	WB: Use at a dilution of 1/50-1/500, incubate for 2 hours at 23C with alkaline phosphatase secondary antibody and BCIP/NBT color development. Detects a band of approximately 33 kDa. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Purity	Ascites
Buffer	Supplied as a liqud in 0.1% sodium azide, before the addition of glycerol to 40%.
Research Area	Transcription Factors
Restrictions	For research use only

Google Scholar	Find more references for clone 2Q248 on Google Scholar™
EMBL Harvester	Search human, mouse or rat genome for antigen 14.03.2003 (Bioinformatic Harvester (EMBL))