14-3-3 gamma antibody

概述


	抗原:	14-3-3 gamma
	抗体类型:	单克隆抗体
	应用范围:	Western Blotting (WB), Immunoprecipitation (IP)
	抗体来源:	Mouse
	规格:	100 µg (1 mg/mL)
	价格:	216,00 € (加上运费和增值税)
	货号:	ABIN131783
	发货时间:	7 to 10 days until shipment

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详细信息: 14-3-3 gamma antibody

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抗原	14-3-3 gamma
免疫原	This antibody was purified from hybridoma (clone KC21) supernatant using protein A agarose. This hybridoma was established by fusion of mouse myeloma cell NS-1 with Balb/c mouse splenocyte immunized with KLH-conjugated human 14-3-3. N-terminal peptide (1 a.a.-12 a.a.).
抗体类型	单克隆抗体
抗体同种型	IgG2a »相配的二抗
描述	The 14-3-3 protein family comprises critical regulatory molecules involved in signaling during cell division, proliferation, and apoptosis. 14-3-3., a subtype of the 14-3-3 family of proteins, was thought to be brain- and neuron-specific. However, 14-3-3. was not neuron-specific but also expressed in astrocytes. Endogenous 14-3-3. proteins in immature astrocytes appeared filamentous and co-localized with filamentous actin (F-actin). 14-3-3. proteins also play a role in cytoskeletal function during the process of cell division and apoptosis in astrocytes in association with actin. This expression is induced in arterial trauma by cytokines, and suggests that this protein may play an important role in progression of vascular proliferative diseases.
克隆	KC21
抗体来源	Mouse
特异性	This antibody reacts with 14-3-3 . (33kDa) on Western blotting.

使用细节: 14-3-3 gamma antibody

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应用范围	Western Blotting (WB), Immunoprecipitation (IP)
应用备注	Western blotting: 0.1 µg / mL for chemiluminescence detection system. Immunoprecipitation: 2 µg / 100 µL of cell extract from 5x10 6 cells. Immunohistochemistry: Not tested. Immunocytochemistry: Not tested. Flow cytometry: Not tested.
浓度	1 mg/mL
缓冲液	100 µg IgG in 100 µL P BS containing 50% glycerol, pH 7.2. Contains no preservatives.
研究领域	Transcription Factors
限制	For Research Use Only

参考文献: 14-3-3 gamma antibody

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参考文献	Chen, Yu: "The association of 14-3-3gamma and actin plays a role in cell division and apoptosis in astrocytes." In: Biochemical and biophysical research communications , Vol. 296, Issue 3, pp. 657-63, 2002/01 (PubMed)

图像: 14-3-3 gamma antibody

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W estern blot analysis of 14-3-3? expression in A431 cells (1), K562 cells (2), NIH /3T3 cells (3), mouse brain (4), PC12 cells (5) and Rat brain (6) using ABIN131783.

图像 14-3-3 gamma antibody (1)

Immunoprecipitation of 14-3-3? from A 431 cells w ith m ouse IgG 2a (1) or K 0203-3 (2) . A fter immunoprecipitated with the antibody, immunocomplex was resolved on SDS -PAGE and immunoblotted with ABIN131783.

图像 14-3-3 gamma antibody (2)