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Détail du produit pour 14-3-3 gamma antibody
14-3-3 gamma antibody
Aperçu
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| Antigène: | 14-3-3 gamma |
| Type anticorps: | Monoclonal |
| Application: | Western Blotting (WB), Immunoprecipitation (IP) |
| Hôte: | Mouse |
| Quantité: | |
| Prix: | 216,00 € (Plus frais de livraison et TVA) |
| Numéro de commande: | ABIN131783 |
| Disponibilité: | 7 to 10 days until shipment |
Description du produit: 14-3-3 gamma antibody
| Antigène | 14-3-3 gamma |
| Immunogène | This antibody was purified from hybridoma (clone KC21) supernatant using protein A agarose. This hybridoma was established by fusion of mouse myeloma cell NS-1 with Balb/c mouse splenocyte immunized with KLH-conjugated human 14-3-3. N-terminal peptide (1 a.a.-12 a.a.). |
| Type anticorps | Monoclonal |
| Isotype | IgG2a »Anticorps secondaires correspondants |
| Description | The 14-3-3 protein family comprises critical regulatory molecules involved in signaling during cell division, proliferation, and apoptosis. 14-3-3., a subtype of the 14-3-3 family of proteins, was thought to be brain- and neuron-specific. However, 14-3-3. was not neuron-specific but also expressed in astrocytes. Endogenous 14-3-3. proteins in immature astrocytes appeared filamentous and co-localized with filamentous actin (F-actin). 14-3-3. proteins also play a role in cytoskeletal function during the process of cell division and apoptosis in astrocytes in association with actin. This expression is induced in arterial trauma by cytokines, and suggests that this protein may play an important role in progression of vascular proliferative diseases. |
| Clone | KC21 |
| Hôte | Mouse |
| Specificité | This antibody reacts with 14-3-3 . (33kDa) on Western blotting. |
Applications: 14-3-3 gamma antibody
| Application | Western Blotting (WB), Immunoprecipitation (IP) |
| Indications d'application | Western blotting: 0.1 µg / mL for chemiluminescence detection system. Immunoprecipitation: 2 µg / 100 µL of cell extract from 5x10 6 cells. Immunohistochemistry: Not tested. Immunocytochemistry: Not tested. Flow cytometry: Not tested. |
| Concentration | 1 mg/mL |
| Buffer | 100 µg IgG in 100 µL P BS containing 50% glycerol, pH 7.2. Contains no preservatives. |
| Domaine de recherche | Transcription Factors |
| Restrictions | For Research Use Only |
Références: 14-3-3 gamma antibody
| Références |
Chen, Yu: "The association of 14-3-3gamma and actin plays a role in cell division and apoptosis in astrocytes." In: Biochemical and biophysical research communications , Vol. 296, Issue 3, pp. 657-63, 2002/01 (PubMed) |
Images: 14-3-3 gamma antibody
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W estern blot analysis of 14-3-3? expression in A431 cells (1), K562 cells (2), NIH /3T3 cells (3), mouse brain (4), PC12 cells (5) and Rat brain (6) using ABIN131783.
Immunoprecipitation of 14-3-3? from A 431 cells w ith m ouse IgG 2a (1) or K 0203-3 (2) . A fter immunoprecipitated with the antibody, immunocomplex was resolved on SDS -PAGE and immunoblotted with ABIN131783. |
Informations supplémentaires: 14-3-3 gamma antibody
| Google Scholar | Trouver des références supplémentaires pour clone KC21 sur Google Scholar™ |
| EMBL Harvester | Recherche humain, souris ou rat génome pour antigène 14-3-3 gamma (Bioinformatic Harvester (EMBL)) |
