Product details for  14-3-3 gamma antibody
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14-3-3 gamma antibody

Overview

Image for 14-3-3 gamma antibody
Antigen: 14-3-3 gamma
Application: Western Blotting (WB), Immunoprecipitation (IP)
Host: Mouse
Quantity: 100 µg (1 mg/mL)
Price: 216,00 €   (Plus shipping costs and VAT)
Order number: ABIN131783
Availability: 7 to 10 days until shipment
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Additional Information:  14-3-3 gamma antibody

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Antigen 14-3-3 gamma
Immunogen This antibody was purified from hybridoma (clone KC21) supernatant using protein A agarose. This hybridoma was established by fusion of mouse myeloma cell NS-1 with Balb/c mouse splenocyte immunized with KLH-conjugated human 14-3-3. N-terminal peptide (1 a.a.-12 a.a.).
Antibody Type
Isotype IgG2a »Matching secondary antibodies
Description The 14-3-3 protein family comprises critical regulatory molecules involved in signaling during cell division, proliferation, and apoptosis. 14-3-3., a subtype of the 14-3-3 family of proteins, was thought to be brain- and neuron-specific. However, 14-3-3. was not neuron-specific but also expressed in astrocytes. Endogenous 14-3-3. proteins in immature astrocytes appeared filamentous and co-localized with filamentous actin (F-actin). 14-3-3. proteins also play a role in cytoskeletal function during the process of cell division and apoptosis in astrocytes in association with actin. This expression is induced in arterial trauma by cytokines, and suggests that this protein may play an important role in progression of vascular proliferative diseases.
Clone KC21
Host Mouse
Specificity This antibody reacts with 14-3-3 . (33kDa) on Western blotting.

Application Details:  14-3-3 gamma antibody

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Application Western Blotting (WB), Immunoprecipitation (IP)
Application Notes Western blotting: 0.1 µg / mL for chemiluminescence detection system. Immunoprecipitation: 2 µg / 100 µL of cell extract from 5x10 6 cells. Immunohistochemistry: Not tested. Immunocytochemistry: Not tested. Flow cytometry: Not tested.
Concentration 1 mg/mL
Buffer 100 µg IgG in 100 µL P BS containing 50% glycerol, pH 7.2. Contains no preservatives.
Research Area Transcription Factors
Restrictions For Research Use Only

Publications:  14-3-3 gamma antibody

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Publications Chen, Yu:
"The association of 14-3-3gamma and actin plays a role in cell division and apoptosis in astrocytes."
In: Biochemical and biophysical research communications , Vol. 296, Issue 3, pp. 657-63, 2002/01 (PubMed)

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W estern blot analysis of 14-3-3? expression in A431 cells (1), K562 cells (2), NIH /3T3 cells (3), mouse brain (4), PC12 cells (5) and Rat brain (6) using ABIN131783.

Image for 14-3-3 gamma antibody (1)

Immunoprecipitation of 14-3-3? from A 431 cells w ith m ouse IgG 2a (1) or K 0203-3 (2) . A fter immunoprecipitated with the antibody, immunocomplex was resolved on SDS -PAGE and immunoblotted with ABIN131783.

Image for 14-3-3 gamma antibody (2)

External Information:  14-3-3 gamma antibody

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Google Scholar Find more references for clone KC21 on Google Scholar™
EMBL Harvester Search human, mouse or rat genome for antigen 14-3-3 gamma (Bioinformatic Harvester (EMBL))