14-3-3 sigma (Human) antibody

Overview

Antigen	14-3-3 sigma
Immunogen	Fusion protein corresponding to full-length human 14-3-3 sigma.
Reactivity	Human, Mouse (Murine), Rat
Antibody Type	Monoclonal
Format	Liquid
Isotype	IgG1 »Matching secondary antibodies
Description	Members of the 14-3-3 family of proteins are highly conserved proteins, localized in neurons, and are axonally transported to the nerve terminals. They are also present, at lower levels, in various other eukaryotic tissues. 14-3-3 proteins appear to play important roles in a variety of signal transduction pathways, including those involved in cell cycle regulation and cell survival. Because 14-3-3 proteins bind to specific phosphoserine-containing sequences they are likely to have an important role in signaling pathways mediated by serine/threonine protein kinases. Evidence indicates 14-3-3 is required for Raf-1 kinase activity and phosphorylation amoung many other functions.
Clone	1-T-28
Host	Mouse
Specificity	ABIN145054 recognizes 14-3-3 sigma and does not crossreact with other 14-3-3 isoforms.

Application	Western Blotting (WB)
Application Notes	WB: Use at a concentration of 0.5 ug/ml. ABIN145054 detects 20ug of reduced EGF-stimulated A431 cell lysate, visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Predicted molecular weight: 30 kDa. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Concentration	1 mg/ml
Purity	Protein G purified
Buffer	Preservative: 0.05% Sodium AzideConstituents: 30% Glycerol, 0.15M Sodium Chloride, 0.1M Tris-glycine. pH 7.4
Restrictions	For research use only

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EMBL Harvester	Search human, mouse or rat genome for antigen 14-3-3 sigma (Bioinformatic Harvester (EMBL))