14-3-3 zeta/delta antibody

概述


	抗原:	14-3-3 zeta/delta
	抗体类型:	多克隆抗体
	应用范围:	Western Blotting (WB)
	目标物种:	Mouse (Murine), Human
	抗体来源:	Rabbit
	规格:	50 µl (5 WB) (相似抗体)
	价格:	87,00 € (加上运费和增值税)
	货号:	ABIN160922
	发货时间:	Ships within 7 to 10 days

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详细信息: 14-3-3 zeta/delta antibody

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抗原	14-3-3 zeta/delta
免疫原	Recombinant (partial), N-terminal
目标物种	Mouse (Murine), Human
抗体类型	多克隆抗体
状态	Purified
抗体同种型	Rabbit IgG »相配的二抗
描述	The 30 kD 14-3-3 cytoplasmic protein participates in signal transduction by binding to phosphoserine-containing proteins and activating protein kinase C. This protein has also been shown to activate tyrosine/ tryptophan hydroxylases in presence of Ca/calmodulin-dependent protein kinase II, regulate insulin sensitivity, inhibit tuberin function, participate in actin cytoskeletal alterations, regulate cell adhesion, and inhibit the pro-apoptotic protein Bad. This protein forms dimers and can be modified by acetylation and phosphorylation. The delta isoform of 14-3-3 is phosphorylated on serine-185, phosphorylation disrupts dimerization. The 14-3-3 protein has been shown to interact with MAPKAPK2, IRS1, PKB/Akt, tuberin, cofilin, LIMK1, ADAM 22, and Bad proteins. The Poly6148 antibody has been shown to be useful for Western blotting of the mouse and human 14-3-3 proten. Distribution: Cytoplasm Function: Mediates signal transduction by binding to phosphoserine-containing proteins. Activates PKC, tyrosine, tryptophan hydroxylases in presence of Ca/calmodulin-dependent protein kinase II. Regulates insulin sensitivity and cell adhesion. Inhibits tuberin function, actin cytoskeleton alterations, and Bad expression Modifications: Phosphorylation, Acetylation Synonyms: Protein kinase C inhibitor protein-1, Phospholipase A2, Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide (YWHAZ) Regulation: Phosphorylation disrupts dimerization Structure: 14-3-3 protein superfamily, dimer, delta isoform is Ser185 phosphorylated, 30 kD
克隆	Poly6148
抗体来源	Rabbit
特异性	MAPKAPK2, IRS1, PKB/Akt, tuberin, cofilin, LIMK1, ADAM 22, Bad.

使用细节: 14-3-3 zeta/delta antibody

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应用范围	Western Blotting (WB)
应用备注	Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 µl per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
纯化	The antibody was purified by antigen-affinity chromatography.
缓冲液	This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 50% glycerol.
贮藏	Upon receipt, store frozen at -20° C.
研究领域	Transcription Factors
限制	For Research Use only

参考文献: 14-3-3 zeta/delta antibody

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参考文献	Johnson, Bernal-Mizrachi, Alejandro et al.: "Insulin protects islets from apoptosis via Pdx1 and specific changes in the human islet proteome." In: Proceedings of the National Academy of Sciences of the United States of America , Vol. 103, Issue 51, pp. 19575-80, 2006/01 (PubMed)

图像: 14-3-3 zeta/delta antibody

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Hela cell extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with rabbit anti-14-3-3 zeta/delta antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system. This antibody detects the 14-3-3 zeta/delta protein (30 kD) as well as a protein of unknown identity with a molecular weight of approximately 60 kD.

图像 14-3-3 zeta/delta antibody (1)