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Détail du produit pour 14-3-3 zeta/delta antibody
14-3-3 zeta/delta antibody
Aperçu
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| Antigène: | 14-3-3 zeta/delta |
| Type anticorps: | Polyclonal |
| Application: | Western Blotting (WB) |
| Reactivité: | Mouse (Murine), Human |
| Hôte: | Rabbit |
| Quantité: | |
| Prix: | 87,00 € (Plus frais de livraison et TVA) |
| Numéro de commande: | ABIN160922 |
| Disponibilité: | Ships within 7 to 10 days |
Description du produit: 14-3-3 zeta/delta antibody
| Antigène | 14-3-3 zeta/delta |
| Immunogène | Recombinant (partial), N-terminal |
| Reactivité | Mouse (Murine), Human |
| Type anticorps | Polyclonal |
| Format | Purified |
| Isotype | Rabbit IgG »Anticorps secondaires correspondants |
| Description | The 30 kD 14-3-3 cytoplasmic protein participates in signal transduction by binding to phosphoserine-containing proteins and activating protein kinase C. This protein has also been shown to activate tyrosine/ tryptophan hydroxylases in presence of Ca/calmodulin-dependent protein kinase II, regulate insulin sensitivity, inhibit tuberin function, participate in actin cytoskeletal alterations, regulate cell adhesion, and inhibit the pro-apoptotic protein Bad. This protein forms dimers and can be modified by acetylation and phosphorylation. The delta isoform of 14-3-3 is phosphorylated on serine-185, phosphorylation disrupts dimerization. The 14-3-3 protein has been shown to interact with MAPKAPK2, IRS1, PKB/Akt, tuberin, cofilin, LIMK1, ADAM 22, and Bad proteins. The Poly6148 antibody has been shown to be useful for Western blotting of the mouse and human 14-3-3 proten. Distribution: Cytoplasm Function: Mediates signal transduction by binding to phosphoserine-containing proteins. Activates PKC, tyrosine, tryptophan hydroxylases in presence of Ca/calmodulin-dependent protein kinase II. Regulates insulin sensitivity and cell adhesion. Inhibits tuberin function, actin cytoskeleton alterations, and Bad expression Modifications: Phosphorylation, Acetylation Synonyms: Protein kinase C inhibitor protein-1, Phospholipase A2, Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide (YWHAZ) Regulation: Phosphorylation disrupts dimerization Structure: 14-3-3 protein superfamily, dimer, delta isoform is Ser185 phosphorylated, 30 kD |
| Clone | Poly6148 |
| Hôte | Rabbit |
| Specificité | MAPKAPK2, IRS1, PKB/Akt, tuberin, cofilin, LIMK1, ADAM 22, Bad. |
Applications: 14-3-3 zeta/delta antibody
| Application | Western Blotting (WB) |
| Indications d'application | Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 µl per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application. |
| Purification | The antibody was purified by antigen-affinity chromatography. |
| Buffer | This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 50% glycerol. |
| Stock | Upon receipt, store frozen at -20° C. |
| Domaine de recherche | Transcription Factors |
| Restrictions | For Research Use only |
Références: 14-3-3 zeta/delta antibody
| Références |
Johnson, Bernal-Mizrachi, Alejandro et al.: "Insulin protects islets from apoptosis via Pdx1 and specific changes in the human islet proteome." In: Proceedings of the National Academy of Sciences of the United States of America , Vol. 103, Issue 51, pp. 19575-80, 2006/01 (PubMed) |
Images: 14-3-3 zeta/delta antibody
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Hela cell extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with rabbit anti-14-3-3 zeta/delta antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system. This antibody detects the 14-3-3 zeta/delta protein (30 kD) as well as a protein of unknown identity with a molecular weight of approximately 60 kD. |
Informations supplémentaires: 14-3-3 zeta/delta antibody
| Google Scholar | Trouver des références supplémentaires pour clone Poly6148 sur Google Scholar™ |
| EMBL Harvester | Recherche humain, souris ou rat génome pour antigène 14-3-3 zeta/delta (Bioinformatic Harvester (EMBL)) |
