14-3-3 zeta/delta antibody

Overview


	Antigen:	14-3-3 zeta/delta
	Antibody Type:	Polyclonal
	Application:	Western Blotting (WB)
	Reactivity:	Mouse (Murine), Human
	Host:	Rabbit
	Quantity:	200 µl (20 WB) (Product variants)
	Price:	260,00 € (Plus shipping costs and VAT)
	Order number:	ABIN160923
	Availability:	Ships within 7 to 10 days

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Additional Information: 14-3-3 zeta/delta antibody

Antigen	14-3-3 zeta/delta
Immunogen	Recombinant (partial), N-terminal
Reactivity	Mouse (Murine), Human
Antibody Type	Polyclonal
Format	Purified
Isotype	Rabbit IgG »Matching secondary antibodies
Description	The 30 kD 14-3-3 cytoplasmic protein participates in signal transduction by binding to phosphoserine-containing proteins and activating protein kinase C. This protein has also been shown to activate tyrosine/ tryptophan hydroxylases in presence of Ca/calmodulin-dependent protein kinase II, regulate insulin sensitivity, inhibit tuberin function, participate in actin cytoskeletal alterations, regulate cell adhesion, and inhibit the pro-apoptotic protein Bad. This protein forms dimers and can be modified by acetylation and phosphorylation. The delta isoform of 14-3-3 is phosphorylated on serine-185, phosphorylation disrupts dimerization. The 14-3-3 protein has been shown to interact with MAPKAPK2, IRS1, PKB/Akt, tuberin, cofilin, LIMK1, ADAM 22, and Bad proteins. The Poly6148 antibody has been shown to be useful for Western blotting of the mouse and human 14-3-3 proten. Distribution: Cytoplasm Function: Mediates signal transduction by binding to phosphoserine-containing proteins. Activates PKC, tyrosine, tryptophan hydroxylases in presence of Ca/calmodulin-dependent protein kinase II. Regulates insulin sensitivity and cell adhesion. Inhibits tuberin function, actin cytoskeleton alterations, and Bad expression Modifications: Phosphorylation, Acetylation Synonyms: Protein kinase C inhibitor protein-1, Phospholipase A2, Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide (YWHAZ) Regulation: Phosphorylation disrupts dimerization Structure: 14-3-3 protein superfamily, dimer, delta isoform is Ser185 phosphorylated, 30 kD
Clone	Poly6148
Host	Rabbit
Specificity	MAPKAPK2, IRS1, PKB/Akt, tuberin, cofilin, LIMK1, ADAM 22, Bad.

Application Details: 14-3-3 zeta/delta antibody

Application	Western Blotting (WB)
Application Notes	Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 µl per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
Purification	The antibody was purified by antigen-affinity chromatography.
Buffer	This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 50% glycerol.
Storage	Upon receipt, store frozen at -20° C.
Research Area	Transcription Factors
Restrictions	For Research Use only

Publications: 14-3-3 zeta/delta antibody

Publications

Johnson, Bernal-Mizrachi, Alejandro et al.:
"Insulin protects islets from apoptosis via Pdx1 and specific changes in the human islet proteome."
In: Proceedings of the National Academy of Sciences of the United States of America , Vol. 103, Issue 51, pp. 19575-80, 2006/01 (PubMed)

Images: 14-3-3 zeta/delta antibody

Hela cell extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with rabbit anti-14-3-3 zeta/delta antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system. This antibody detects the 14-3-3 zeta/delta protein (30 kD) as well as a protein of unknown identity with a molecular weight of approximately 60 kD.

Image for 14-3-3 zeta/delta antibody (1)

Product variants

14-3-3 zeta/delta antibody (Format: Purified, Quantity: 50 µl (5 WB))

External Information: 14-3-3 zeta/delta antibody

Google Scholar	Find more references for clone Poly6148 on Google Scholar™
EMBL Harvester	Search human, mouse or rat genome for antigen 14-3-3 zeta/delta (Bioinformatic Harvester (EMBL))