BASKET
HELP
Product details for 14-3-3b/e (Human) antibody
14-3-3b/e (Human) antibody
Overview
| Antigen: | 14-3-3b/e |
| Antibody Type: | Monoclonal |
| Application: | Western Blotting (WB) |
| Reactivity: | Human, Mouse (Murine), Rat |
| Host: | Mouse |
| Quantity: | |
| Price: | 216,00 € (Plus shipping costs and VAT) |
| Order number: | ABIN131845 |
| Availability: | 7 to 10 days until shipment |
Additional Information: 14-3-3b/e (Human) antibody
| Antigen | 14-3-3b/e |
| Immunogen | This antibody was purified from mouse ascites fluid using Protein-A Sepharose. This hybridoma was established by fusion of mouse myeloma SP2/0-Ag14 with Balb/c splenocyte immunized with recombinant GST- human 14-3-3 ß fusion protein |
| Reactivity | Human, Mouse (Murine), Rat |
| Antibody Type | Monoclonal |
| Format | Lyophilised |
| Isotype | IgG2b »Matching secondary antibodies |
| Description | 14-3-3 proteins are highly conserved ubiquitous proteins and are expressed in broad range of tissues and cell types. There are at least seven mammalian 14-3-3 isoforms, named a-. , that form homo- and heterodimer. 14-3-3 proteins bind to variety proteins and play important roles in signal transduction and progression through cell cycle. For example, several kinases including Raf, PKC, c-Bcr and PI3-kinase bind to 14-3-3, and as a consequence may have their kinase activity altered by this binding depending on the experimental conditions used. |
| Clone | 3C8 |
| Host | Mouse |
| Specificity | This antibody reacts with recombinant 14-3-3 ß , e and . on Western blotting. This antibody detects 30 KD of human 14-3-3 on Western blotting with total cell lysate from HeLa cell. This antibody also reacts with mouse and rat 14-3-3 on western blotting with mouse and rat brain extract respectively. |
Application Details: 14-3-3b/e (Human) antibody
| Application | Western Blotting (WB) |
| Application Notes | 7) Incubate in SDS PAGE & Western Blotting: Horseradish Peroxidase conjugated goat 1) Boil all samples for 3~5 minutes. Load 10: anti-mouse (MBL, code: 330) diluted 1:3000 in PBS, l of cell: 8) Wash the membrane 3 times with PBS, 0.05% Tween20 lysate or tissue homogenate (5~20: for 10 minutes per wash. Western blotting: 1-10 µ g/ml Details are provided concerning the following research applications. |
| Buffer | This antibody is lyophilized form. Prepare a stock solution by dissolving the lyophilized antibody in 100 µ l of distilled water. After reconstitution, the IgG concentration should be 1.0 mg/ml in PBS (pH7.2) / 1% Sucrose / 0.1% Sodium azide. *When dispos |
| Research Area | Transcription Factors |
| Restrictions | For Research Use Only |
Publications: 14-3-3b/e (Human) antibody
| Publications |
Aitken: "14-3-3 and its possible role in co-ordinating multiple signalling pathways." In: Trends in cell biology , Vol. 6, Issue 9, pp. 341-7, 2004/01 (PubMed) |
browse antibodies by
Antigens
Secondary Antibodies
Research areas
Clones
Hosts
Applications
Reactivity
Supplier
Antigens
Secondary Antibodies
Research areas
Clones
Hosts
Applications
Reactivity
Supplier
