Product details for  15S Mg2+-ATPase (Xenopus) antibody
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15S Mg2+-ATPase (Xenopus) antibody

Overview

Antigen: ATPase (15S Mg) p97 subunit
Antibody Type: Monoclonal
Application: Western Blotting (WB)
Reactivity: Human, Rabbit, Rat, Xenopus
Host: Mouse
Quantity: 0.5 ml
Price: 280,00 €   (Plus shipping costs and VAT)
Order number: ABIN125949
Availability: 3 to 10 days until shipment
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Additional Information:  15S Mg2+-ATPase (Xenopus) antibody

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Antigen ATPase (15S Mg) p97 subunit
Immunogen 15S Mg2+ ATPase complex purified from Xenopus laevis ovary
Reactivity Human, Rabbit, Rat, Xenopus
Antibody Type Monoclonal
Format Supernatant
Isotype IgG1 »Matching secondary antibodies
Clone 58-13-3
Host Mouse
Specificity P97 ATPase is implicated in homotypic membrane fusion events and is related to the N-ethyl maleimide-sensitive fusion protein NSF/Sec18p. The antibody reacts with the p97 subunit of 15S Mg2+-ATPase.

Application Details:  15S Mg2+-ATPase (Xenopus) antibody

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Application Western Blotting (WB)
Application Notes Immunoblotting: 1:1000 – 1:2000. Not recommended for immunofluorescence and immunoprecipitation. Dilution buffer: 0.15 M PBS with 0.1% BSA and 0.1% Na-Azide (ECL: 5% milk powder in TBST). Positive control: Immunoblots with protein extracts from all vertebrate tissues and cells. Other applications not tested. Optimal dilutions of this antibody are dependent on conditions and should be determined by the user.
Buffer This antibody is supplied as supernatant concentrate.
Storage At 2-8°C. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Restrictions For Research Use only

Publications:  15S Mg2+-ATPase (Xenopus) antibody

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Publications Acharya, Jacobs, Peters et al.:
"The formation of Golgi stacks from vesiculated Golgi membranes requires two distinct fusion events."
In: Cell , Vol. 82, Issue 6, pp. 895-904, 1995/01 (PubMed)

Peters, Harris, Lustig et al.:
"Ubiquitous soluble Mg(2+)-ATPase complex. A structural study."
In: Journal of molecular biology , Vol. 223, Issue 2, pp. 557-71, 1992/01 (PubMed)

Peters, Walsh, Franke:
"An abundant and ubiquitous homo-oligomeric ring-shaped ATPase particle related to the putative vesicle fusion proteins Sec18p and NSF."
In: The EMBO journal , Vol. 9, Issue 6, pp. 1757-67, 1990/01 (PubMed)

Rabouille, Levine, Peters et al.:
"An NSF-like ATPase, p97, and NSF mediate cisternal regrowth from mitotic Golgi fragments."
In: Cell , Vol. 82, Issue 6, pp. 905-14, 1995/01 (PubMed)

External Information:  15S Mg2+-ATPase (Xenopus) antibody

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Google Scholar Find more references for clone 58-13-3 on Google Scholar™
EMBL Harvester Search human, mouse or rat genome for antigen ATPase (15S Mg) p97 subunit (Bioinformatic Harvester (EMBL))