Product details for  15S Mg2+-ATPase (Xenopus) antibody
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15S Mg2+-ATPase (Xenopus) antibody

Overview

Antigen: ATPase (15S Mg) p97 subunit
Antibody Type: Monoclonal
Application: Western Blotting (WB)
Reactivity: Human, Rabbit, Rat, Xenopus
Host: Mouse
Quantity: 50 ug
Price: 300,00 €   (Plus shipping costs and VAT)
Order number: ABIN125950
Availability: 3 to 10 days until shipment
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Additional Information:  15S Mg2+-ATPase (Xenopus) antibody

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Antigen ATPase (15S Mg) p97 subunit
Immunogen 15S Mg2+ ATPase complex purified from Xenopus laevis ovary
Reactivity Human, Rabbit, Rat, Xenopus
Antibody Type Monoclonal
Format Purified
Isotype IgG1 »Matching secondary antibodies
Clone 58-13-3
Host Mouse
Specificity P97 ATPase is implicated in homotypic membrane fusion events and is related to the N-ethyl maleimide-sensitive fusion protein NSF/Sec18p. The antibody reacts with the p97 subunit of 15S Mg2+-ATPase. All vertebrate cells and tissues tested so far: e.g. Xenopus laevis, rabbit, rat, human, does not crossreact with the corresponding protein in budding yeast (Cdc48p).

Application Details:  15S Mg2+-ATPase (Xenopus) antibody

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Application Western Blotting (WB)
Application Notes Immunoblotting. Not recommended for immunofluorescence and immunoprecipitation. Positive control: Immunoblots with protein extracts from all vertebrate tissues and cells. Working dilution: When reconstituted with 1 ml distilled water, dilute further 1:50 – 1:200 for Western Blot (ECL method). Dilution buffer: ECL: 5% milk powder in TBST. Other applications not tested. Optimal dilutions of this antibody are dependent on conditions and should be determined by the user.
Buffer This Protein G affinity purified antibody is supplied as lyophilisate. Reconstitute in 1 ml dist. water (final solution contains 0.09 % NaN3, 0.5% BSA in PBS buffer, pH 7.4).
Storage At 2-8°C one year after reconstitution. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Restrictions For Research Use only

Publications:  15S Mg2+-ATPase (Xenopus) antibody

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Publications Acharya, Jacobs, Peters et al.:
"The formation of Golgi stacks from vesiculated Golgi membranes requires two distinct fusion events."
In: Cell , Vol. 82, Issue 6, pp. 895-904, 1995/01 (PubMed)

Peters, Harris, Lustig et al.:
"Ubiquitous soluble Mg(2+)-ATPase complex. A structural study."
In: Journal of molecular biology , Vol. 223, Issue 2, pp. 557-71, 1992/01 (PubMed)

Peters, Walsh, Franke:
"An abundant and ubiquitous homo-oligomeric ring-shaped ATPase particle related to the putative vesicle fusion proteins Sec18p and NSF."
In: The EMBO journal , Vol. 9, Issue 6, pp. 1757-67, 1990/01 (PubMed)

Rabouille, Levine, Peters et al.:
"An NSF-like ATPase, p97, and NSF mediate cisternal regrowth from mitotic Golgi fragments."
In: Cell , Vol. 82, Issue 6, pp. 905-14, 1995/01 (PubMed)

External Information:  15S Mg2+-ATPase (Xenopus) antibody

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Google Scholar Find more references for clone 58-13-3 on Google Scholar™
EMBL Harvester Search human, mouse or rat genome for antigen ATPase (15S Mg) p97 subunit (Bioinformatic Harvester (EMBL))