AKT Phospho specific pS474 (Human) antibody

Overview


	Antigen:	AKT Phospho specific pS474
	Antibody Type:	Polyclonal
	Application:	Immunohistochemistry (IHC), ELISA, Western Blotting (WB), Immunoprecipitation (IP)
	Reactivity:	Human, Bovine, Mouse (Murine), Rat
	Host:	Rabbit
	Quantity:	100ug (1.7 mg/ml (by UV absorbance at...)
	Price:	338,33 $ (Plus shipping costs and VAT, Invoice in EUR)
	Order number:	ABIN94784
	Availability:	7 days until shipment

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Additional Information: AKT Phospho specific pS474 (Human) antibody

Antigen	AKT Phospho specific pS474
Immunogen	.
Reactivity	Human, Bovine, Mouse (Murine), Rat
Antibody Type	Polyclonal
Format	Liquid (sterile filtered)
Isotype	IgG »Matching secondary antibodies
Host	Rabbit

Application Details: AKT Phospho specific pS474 (Human) antibody

Application	Immunohistochemistry (IHC), ELISA, Western Blotting (WB), Immunoprecipitation (IP)
Concentration	1.7 mg/ml (by UV absorbance at 280 nm)
Purity	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2Stabilizer: None Preservative: 0.01% (w/v) Sodium Azide
Purification	This product was prepared from monospecific antiserum by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities. Assay by immunoelectro-phoresis resulted in a single precipitin arc against anti-Rabbit Serum. This antibody is specific for phosphorylated human AKT. Minimal reactivity occurs against non-phosphorylated AKT. Reactivity against AKT from other species may occur but has not yet been tested.
Storage	Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.
Research Area	Kinases/Phosphatases
Restrictions	For Research Use only

Images: AKT Phospho specific pS474 (Human) antibody

Immunohistochemistry. Rabbit anti-AKT pS473 was used at a 1:100 dilution to detect AKT by immunohistochemistry in human breast tumor tissue. The staining is much stronger than the weak basal level of phosphorylation in normal breast. antibodies-online's Rabbit anti-AKT pS473 antibody was used with no pretreatment of tissue. Signal was developed using Dako's Techmate streptavidinbiotin reagents. Tissue was formalin-fixed and paraffin embedded.

Image for AKT Phospho specific pS474 (Human) antibody (1)

Immunohistochemistry. This image is a higher magnification of the breast tumor image above.

Image for AKT Phospho specific pS474 (Human) antibody (2)

Image for AKT Phospho specific pS474 (Human) antibody (3)

External Information: AKT Phospho specific pS474 (Human) antibody

Google Scholar	Find more references for antigen AKT Phospho specific pS474 on Google Scholar™
EMBL Harvester	Search human, mouse or rat genome for antigen AKT Phospho specific pS474 (Bioinformatic Harvester (EMBL))