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Product details for APOM (Dog (Canine)) antibody
APOM (Dog (Canine)) antibody
Overview
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| Antigen: | Apolipoprotein M (Apo-M) |
| Antibody Type: | Polyclonal |
| Reactivity: | Dog (Canine), Human |
| Host: | Goat |
| Quantity: | |
| Price: | 200,00 € (Plus shipping costs and VAT) |
| Order number: | ABIN190884 |
| Availability: | 3 to 10 days until shipment |
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Additional Information: APOM (Dog (Canine)) antibody
| Antigen | Apolipoprotein M (Apo-M) |
| Gene ID | 55937 |
| Immunogen | Peptide with sequence PRNQEACELSNN, from the C Terminus of the protein sequence according to NP_061974.2 |
| Reactivity | Dog (Canine), Human |
| Antibody Type | Polyclonal |
| Format | Aff - Purified |
| Description | Official Gene Symbol: APOM Accession Number(s): NP_061974.2. . Principal Names: APOM, apolipoprotein M , G3a, HSPC336, MGC22400, NG20 , NG20-like protein, G3a, NG20. |
| Host | Goat |
| Specificity | Expected reactivity from sequence similarity: Human, Dog |
Application Details: APOM (Dog (Canine)) antibody
| Application Notes | Peptide ELISA: antibody detection limit dilution 1:8,000. Western Blot: Preliminary experiments gave an approx 50kDa band in Human Kidney and Liver lysates after 0.2µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 21.3kDa according to NP_061974.2. We do note that ApoM is a glycoprotein which makes a higher size band to be expected. The 50kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?Immunohistochemistry: In paraffin embedded Human Liver shows strong stain of cytoplasm in select hepatocytes. Recommended concentration, 3-6µg/ml. |
| Format | Aff - Purified |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. |
| Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
| Restrictions | For Research Use Only |
External Information: APOM (Dog (Canine)) antibody
| Google Scholar | Find more references for antigen Apolipoprotein M (Apo-M) on Google Scholar™ |
| EMBL Harvester | Search human, mouse or rat genome for antigen Apolipoprotein M (Apo-M) (Bioinformatic Harvester (EMBL)) |
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