ATAD5 (Mouse (Murine)) antibody

Overview

Antigen	ATAD5
Gene ID	237877 (mouse)
Immunogen	Peptide with sequence C-QSKDQPLRKSQK, from the internal region of the protein sequence according to NP_001025027.1
Reactivity	Mouse (Murine), Rat
Antibody Type	Polyclonal
Format	Aff - Purified
Description	Official Gene Symbol: Atad5 Accession Number(s): NP_001025027.1. . Principal Names: Atad5, ATPase family, AAA domain containing 5, C17orf41, chromosome 17 open reading frame 41, FLJ12735, FRAG1 , chromosome fragility associated gene 1, hypothetical protein LOC237877, RP23-328O2.1, C130052G03Rik.
Host	Goat
Specificity	Expected reactivity from sequence similarity: Mouse, Rat

Application Notes	Peptide ELISA: antibody detection limit dilution 1:64,000. Western Blot: Preliminary experiments gave an approx 60kDa band in lysates of cell lines NIH3T3 and NSO after 0.01µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 204kDa according to NP_001025027.1. The 60kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Format	Aff - Purified
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer	0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Storage	Aliquot and store at -20°C. Minimize freezing and thawing.
Restrictions	For Research Use Only

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