ATM Protein Kinase S1981 (Human) antibody

Overview


	Antigen:	ATM Protein Kinase S1981
	Antibody Type:	Polyclonal
	Application:	Immunofluorescense (IF), ELISA, Western Blotting (WB), Immunoprecipitation (IP)
	Reactivity:	Human, Mouse (Murine), Rat, Sheep (Ovine)
	Host:	Mouse
	Quantity:	100ug (1.0 mg/ml (by UV absorbance at...)
	Price:	428,33 $ (Plus shipping costs and VAT, Invoice in EUR)
	Order number:	ABIN95421
	Availability:	7 days until shipment

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Additional Information: ATM Protein Kinase S1981 (Human) antibody

Antigen	ATM Protein Kinase S1981
Immunogen	.
Reactivity	Human, Mouse (Murine), Rat, Sheep (Ovine)
Antibody Type	Polyclonal
Format	Liquid (sterile filtered)
Isotype	IgG »Matching secondary antibodies
Host	Mouse

Application Details: ATM Protein Kinase S1981 (Human) antibody

Application	Immunofluorescense (IF), ELISA, Western Blotting (WB), Immunoprecipitation (IP)
Concentration	1.0 mg/ml (by UV absorbance at 280 nm)
Purity	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2Stabilizer: None Preservative: 0.01% (w/v) Sodium Azide Clone: 10H11.E12 (IgG1) Fusion Partner: Sp2/0 mouse myeloma
Purification	This Protein A Purified Mab antibody is directed against human ATM and is useful in determining its presence in various assays. This monoclonal anti-ATM antibody recognizes the phosphorylated epitope in native and over-expressed proteins found in various tissues and extracts. By ELISA reactivity against SLAFEEGSpQSTTISS at a 1:1600 dilution shows an absorbance >3.000, whereas reactivity against SLAFEEGSQSTTISS shows and absorbance of 0.145. Reactivity is observed against human and mouse ATM. Cross reactivity with ATM from other mammalian sources has not been tested.
Storage	Store vial at -20° C. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use. Expiration date is one (1) year from date of restoration.
Research Area	Kinases/Phosphatases
Restrictions	For Research Use only

Images: ATM Protein Kinase S1981 (Human) antibody

Western blot of gamma irradiated (left lane) and mockirradiated (right lane) human Hela cell lysate. antibodies-online's Protein A Purified Mab anti-ATM Protein Kinase pS1981 detects a 390 kDa band corresponding to phosphorylated ATM. The antibody does not react with non-phosphorylated ATM present in the control lane. The protein was transferred to nitrocellulose using standard methods. After blocking the membrane was probed with the primary antibody diluted to 10 ug/ml for 1 h at room temperature followed by washes and reaction with a 1:1000 dilution of IRDye

Image for ATM Protein Kinase S1981 (Human) antibody (1)

External Information: ATM Protein Kinase S1981 (Human) antibody

Google Scholar	Find more references for antigen ATM Protein Kinase S1981 on Google Scholar™
EMBL Harvester	Search human, mouse or rat genome for antigen ATM Protein Kinase S1981 (Bioinformatic Harvester (EMBL))