ATM antibody

Overview


	Antigen:	ATM
	Antibody Type:	Polyclonal
	Application:	Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
	Host:	Rabbit
	Quantity:	100 µg (1 mg/mL)
	Price:	311,19 $ (Plus shipping costs , $13.07 dry ice fee and VAT, Invoice in EUR)
	Order number:	ABIN132051
	Availability:	7 to 10 days until shipment

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Additional Information: ATM antibody

Antigen	ATM
Immunogen	This antibody was purified from rabbit serum using an affinity chromatography. The rabbit was immunized with thyroglobulin (TG) conjugated CFIKKPFDRGEVESMEDDTNGN peptide.
Antibody Type	Polyclonal
Description	ATM (ataxia telangiectasia mutated) encodes a 350–370 kDa protein. The ATM protein belongs to a family of protein kinases that are involved in cell cycle control and DNA damage response. ATM kinase activity is enhanced immediately after exposure of cells to DNA double strand breaks. The C-terminal region of the protein has extensive homology to the catalytic domain of PI-3 (phosphatydilinositol-3) kinase, FAT (FRAP/ATM/TRAPP), and FAT-C (FAT-carboxy-terminal). ATM activates p53, increasing p21Cip1 levels, thus blocking activation of cdk2. That results in blockage of the G1/S transition.
Host	Rabbit
Specificity	This antibody reacts with ATM (350-370 kDa) by Western blotting.

Application Details: ATM antibody

Application	Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
Application Notes	Western blotting: 1 µg / mL for chemiluminescence detection system. Immunoprecipitation: 5 µg / 300 µL of cell extract from 5x10 6 cells. Immunohistochemistry: 10 µg / mL. Immunocytochemistry: 10 µg / mL. Flow Cytometry: Not tested.
Concentration	1 mg/mL
Buffer	100 µg IgG in 100 µL PBS containing 50% glycerol, pH 7.2. Contains no preservatives.
Restrictions	For Research Use Only

Publications: ATM antibody

Publications

Lai, Chun, Nahas et al.:
"Correction of ATM gene function by aminoglycoside-induced read-through of premature termination codons."
In: Proceedings of the National Academy of Sciences of the United States of America , Vol. 101, Issue 44, pp. 15676-81, 2004/01 (PubMed)

Dong, Zhu, Tamada et al.:
"B7-H1, a third member of the B7 family, co-stimulates T-cell proliferation and interleukin-10 secretion."
In: Nature medicine , Vol. 5, Issue 12, pp. 1365-9, 1999/01 (PubMed)

Driscoll, Hassenbein, Howard et al.:
"Cloning, expression, and functional characterization of rat MIP-2: a neutrophil chemoattractant and epithelial cell mitogen."
In: Journal of leukocyte biology , Vol. 58, Issue 3, pp. 359-64, 1995/01 (PubMed)

Images: ATM antibody

Immunohistochemical detection of ATM on paraffin embedded section of Human stomach cancer cells with ABIN132051.

Image for ATM antibody (1)

Western blot analysis of ATM expression in HeLa cells using ABIN132051.

Image for ATM antibody (2)

Immunoprecipitation of ATM from HeLa cells with Rabbit IgG (1) or ABIN132051 (2). After immunoprecipitated with the antibody, immunocomplex was resolved on SDS-PAGE and immunoblotted with ABIN132051.

Image for ATM antibody (3)

External Information: ATM antibody

Google Scholar	Find more references for antigen ATM on Google Scholar™
EMBL Harvester	Search human, mouse or rat genome for antigen ATM (Bioinformatic Harvester (EMBL))