Abi1 (Abl-Interactor-1)/E3B1 (H) antibody

Overview


	Antigen:	Abi1 (Abl-Interactor-1)/E3B1
	Antibody Type:	Monoclonal
	Application:	Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (IHC)
	Reactivity:	Human, Mouse (Murine), Hamster
	Host:	Mouse
	Quantity:	100 µg (1 mg/mL)
	Price:	383,23 $ (Plus shipping costs , $13.07 dry ice fee and VAT, Invoice in EUR)
	Order number:	ABIN130666
	Availability:	7 to 10 days until shipment

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Additional Information: Abi1 (Abl-Interactor-1)/E3B1 (H) antibody

Antigen	Abi1 (Abl-Interactor-1)/E3B1
Immunogen	This antibody was purified from hybridoma (clone 1G9) supernatant using protein A agarose. This hybridoma was established by fusion of mouse myeloma cell Sp/O with Balb/c mouse splenocyte immunized with a human recombinant Abi-1 expressed in E. coli.
Reactivity	Human, Mouse (Murine), Hamster
Antibody Type	Monoclonal
Isotype	IgG1 »Matching secondary antibodies
Description	Abl interactor (Abi) proteins are c-Abl-binding proteins that bind to both the Src homology 3 (SH3) domain and the C-terminal proline-rich regions of Abl kinase through dual SH3-PXXP interactions. Two highly related genes, abi-1 and abi-2, were cloned. In addition to the interaction with Abl kinase, Abi proteins also interact with other signaling molecules. Recently, it has been reported that Abi-1 dramatically promoted c-Abl-mediated tyrosine (Tyr 296 ) phosphorylation of Mena [mammalian homologue of Drosophila Enabled (Ena)] by interacting with both proteins.
Clone	1G9
Host	Mouse
Specificity	This antibody reacts with Abi-1 (65 kDa) on Western blotting.

Application Details: Abi1 (Abl-Interactor-1)/E3B1 (H) antibody

Application	Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (IHC)
Application Notes	Western blotting: 1 µg / mL for chemiluminescence detection system. Immunoprecipitation: 2 µg / 200 µL of cell extract from 5x10 6 cells. Immunohistochemistry: 5 µg / mL Heat treatment is necessary for paraffin embedded sections. Microwave oven: 2 times for 10 minutes each in citrate buffer (pH 6.5). Immunocytochemistry: Not tested. This antibody was used in immunocytochemistry in ref. 1.
Concentration	1 mg/mL
Buffer	100 µg IgG in 100 µL of PBS containing 50% glycerol, pH 7.2. No preservative is contained.
Restrictions	For Research Use Only

Publications: Abi1 (Abl-Interactor-1)/E3B1 (H) antibody

Publications

Yang, Ta, Pessah et al.:
"Functional defects in six ryanodine receptor isoform-1 (RyR1) mutations associated with malignant hyperthermia and their impact on skeletal excitation-contraction coupling."
In: The Journal of biological chemistry , Vol. 278, Issue 28, pp. 25722-30, 2003/01 (PubMed)

Images: Abi1 (Abl-Interactor-1)/E3B1 (H) antibody

Western blot analysis of Abi-1 expression in HeLa cells (1), 293T cells (2), Raji cells (3), Jurkat cells (4), A431 cells (5), U251 cells (6), KG1 cells (7), NIH/3T3 cells (8), WR19L cells (9) and CHO cells (10) using ABIN130666

Image for Abi1 (Abl-Interactor-1)/E3B1 (H) antibody (1)

Immunohistochemical detection of Abi - 1 on paraffin embedded section of a human lung cancer with ABIN130666.

Image for Abi1 (Abl-Interactor-1)/E3B1 (H) antibody (2)

External Information: Abi1 (Abl-Interactor-1)/E3B1 (H) antibody

Google Scholar	Find more references for clone 1G9 on Google Scholar™
EMBL Harvester	Search human, mouse or rat genome for antigen Abi1 (Abl-Interactor-1)/E3B1 (Bioinformatic Harvester (EMBL))