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Product details for Acetylcholine Esterase Biotin (Bovine) antibody
Acetylcholine Esterase Biotin (Bovine) antibody
Overview
| Antigen: | Acetylcholine Esterase [Bovine Erythrocytes] |
| Antibody Type: | Polyclonal |
| Application: | Enzyme Immunoassay (EIA) |
| Conjugate: | Biotin |
| Reactivity: | Bovine |
| Host: | Goat |
| Quantity: | |
| Price: | 489,84 $ (Plus shipping costs and VAT, Invoice in EUR) |
| Order number: | ABIN116885 |
| Availability: | Ships in 3 to 10 days |
Additional Information: Acetylcholine Esterase Biotin (Bovine) antibody
| Antigen | Acetylcholine Esterase [Bovine Erythrocytes] |
| Immunogen | Acetyl Cholinesterase [Bovine Erythrocytes] |
| Reactivity | Bovine |
| Antibody Type | Polyclonal |
| Host | Goat |
| Specificity | This product is an IgG fraction antibody purified from monospecific antiserum by a multistep process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Acetyl Cholinesterase [Bovine Erythrocytes]. Cross reactivity against Acetyl Cholinesterase from other sources is unknown. |
Application Details: Acetylcholine Esterase Biotin (Bovine) antibody
| Application | Enzyme Immunoassay (EIA) |
| Application Notes | Suitable for immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency. This product has been assayed against 1.0 ug of Acetyl Cholinesterase in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2’-azino-bis-[3- ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:2,000 is suggested for this product. Other applications not tested. Optimal dilutions of this antibody are dependent on conditions and should be determined by the user. |
| Conjugate | Biotin |
| Concentration | 10.0 mg/ml (by UV absorbance at 280 nm) |
| Buffer | This antibody was lyophilised from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/ml Bovine Serum Albumin (BSA) IgG and Protease free as stabilizer and 0.01% (w/v) Sodium Azide as preservative. Label: Biotinamidocaproate N-Hydroxys |
| Storage | Store vial at 4° C prior to restoration. Restore with 1.0 ml of deionized water (or equivalent). For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of restoration. |
| Research Area | Neurotransmitters |
| Restrictions | Research only |
Publications: Acetylcholine Esterase Biotin (Bovine) antibody
| Publications |
Wilchek, Bayer: "Labeling glycoconjugates with hydrazide reagents." In: Methods in enzymology , Vol. 138, pp. 429-42, 1987/01 (PubMed) |
External Information: Acetylcholine Esterase Biotin (Bovine) antibody
| Google Scholar | Find more references for antigen Acetylcholine Esterase [Bovine Erythrocytes] on Google Scholar™ |
| EMBL Harvester | Search human, mouse or rat genome for antigen Acetylcholine Esterase [Bovine Erythrocytes] (Bioinformatic Harvester (EMBL)) |
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