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Product details for Acetylcholinesterase (Bovine) antibody
Acetylcholinesterase (Bovine) antibody
Overview
| Antigen: | Acetylcholine Esterase |
| Antibody Type: | Monoclonal |
| Application: | Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP) |
| Reactivity: | Bovine, Cat (Feline), Guinea Pig, Human, Rabbit |
| Host: | Mouse |
| Quantity: | |
| Price: | 504,25 $ (Plus shipping costs and VAT, Invoice in EUR) |
| Order number: | ABIN152687 |
| Availability: | Ships in 7 to 10 days |
Additional Information: Acetylcholinesterase (Bovine) antibody
| Antigen | Acetylcholine Esterase |
| Immunogen | Purified human cerebellar acetylcholinesterase |
| Reactivity | Bovine, Cat (Feline), Guinea Pig, Human, Rabbit |
| Antibody Type | Monoclonal |
| Format | Ascites |
| Isotype | IgG2b »Matching secondary antibodies |
| Description | Cholinergic neurotransmission occurs in motor, autonomic and central nervoussynapses and requires very rapid inactivation of its transmitter, acetylcholine (ACh). Acetylcholinesterase (AChE) rapidly hydrolyzes ACh to acetate and choline, therebyinactivating it. AChE is found in the neuromuscular junction anchored to the basallamina which runs between the nerve terminal and muscle membrane. AChE is alsofound outside the nervous and neuromuscular system in blood, lymph, germ and livercells suggesting a role for AChE not related to cholinergic transmission. Another lessspecific cholinesterase, butyrylcholinesterase (BChE), seems to contribute to theregulation of the ACh concentration in the synaptic cleft. |
| Clone | HR2 |
| Host | Mouse |
| Specificity | This antibody does not detect butyrylcholinesterase (BChE). Cross-reacts with Human, Rabbit, Cow, Cat and Guinea pig. Not yet tested in otherspecies. |
Application Details: Acetylcholinesterase (Bovine) antibody
| Application | Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP) |
| Application Notes | Suggested working dilutions *ELISA 1:50,immunohistochemistry -F,immunoprecipitation , * Investigator should determine optimal working dilutions. Immunohistochemistry: use at a dilution of 1/50, ELISA, Immunoprecipitation.Immunohistochemical staining of AChE in human brain samples results in staining ofnerve fibers and terminals. This antibody cannot be used in Western blot to detectAChE. This antibody is not suitable for FACS. * Other applications have not been tested. |
| Buffer | PBS with 0.05% sodium azide 0.2 ml Ascites Mouse ascites. |
| Storage | Aliquot and store at -20 degrees C. Avoid repeated freeze / thaw cycles. |
| Research Area | Neurotransmitters |
| Restrictions | For Research Use only |
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