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应用范围
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ELISA, Western Blotting (WB), Immunoprecipitation (IP)
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应用备注
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Dilution: This product has been assayed against 1.0 ug of Acid Phosphatase [Potato] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified antiRabbit IgG [HampL] [Goat] code 6111302 and [ABTS [22'azinobis[3ethylbenthiazoline6sulfonic acid]] code ABTS100 as a substrate for 30 minutes at room temperature. A working dilution of 1/2000 to 1/8000 of the reconstitution concentration is suggested for this product. Optimal titers for other applications should be determined by the researcher. Notice: suitable for immunoblotting [western or dot blot] ELISA immunoprecipitation and most immunological methods requiring high titer and specificity. Suitable for immunoblotting (western or dot blot), ELISA, immunoprecipitation and mostimmunological methods requiring high titer and specificity.
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浓度
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90.0 mg/ml (by Refractometry)
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纯度
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0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2Stabilizer: None Preservative: 0.01% (w/v) Sodium Azide
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纯化
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This product was prepared from monospecific antiserum by a delipidation anddefibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partiallypurified Acid Phosphatase [Potato]. Cross reactivity against Acid Phosphatase from other tissues and speciesmay occur but have not been specifically determined.
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贮藏
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Store vial at 4° C prior to restoration. Restore with 2.0 ml of deionized water (orequivalent). Centrifuge product if not completely clear after standing at room temperature. This product is stablefor several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquotcontents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) yearfrom date of restoration.
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研究领域
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Serum/Plasma Proteins, Kinases/Phosphatases
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限制
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For Research Use only
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