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Product details for Actin antibody
Actin antibody
Overview
| Antigen: | Actin |
| Antibody Type: | Monoclonal |
| Application: | ELISA, Immunocytochemistry (ICC), Immunohistochemistry ((Frozen Sections) (IHC) (fro)), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB) |
| Host: | Mouse |
| Quantity: | |
| Price: | 266,00 € (Plus shipping costs and VAT) |
| Order number: | ABIN146209 |
| Availability: | 7 to 10 days until shipment |
Additional Information: Actin antibody
| Antigen | Actin |
| Immunogen | Chicken gizzard actin |
| Antibody Type | Monoclonal |
| Format | Liquid |
| Isotype | IgG1 »Matching secondary antibodies |
| Description | The two major cytoskeletal proteins implicated in cell motility are actin and myosin. Actin and myosin are constituents of many cell types and are involved in a myriad of cellular processes including locomotion, secretion, cytoplasmic streaming, phagocytosis and cytokinesis. Although actin is one of the most conserved eukaryotic proteins, it is expressed in mammals and birds as at least six isoforms characterized by electrophoresis and amino acid sequence analysis. Four of them represent the differentiation markers of muscle tissues and two are found in practically all cells. |
| Clone | 2Q1055 |
| Host | Mouse |
| Specificity | ABIN146209 recongizes all six isoforms of vertebrate actin, globular (G) and fillimentous (F) forms, it is a pan-actin antibody that binds to an epitope in a highly conserved region of actin. ABIN146209 labels myotubes and stains myoblasts and fibroblasts, it |
Application Details: Actin antibody
| Application | ELISA, Immunocytochemistry (ICC), Immunohistochemistry ((Frozen Sections) (IHC) (fro)), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB) |
| Application Notes | ELISA: Use at an assay dependent dilution. In ELISA, shows a strong reactivity with cytoplasmic actin and significant binding to gizzard, skeletal, arterial and cardiac actins. Also shows significant binding to both Dictyostelium discoidum and Physarum polycephalum in ELISA. ICC: Use at a concentration of 10 ug/ml on methanol fixed mouse 3T3 cells. IHC-P: Use at a concentration of 10 ug/ml. WB: Use at a concentration of 1-20 ug/ml. On muscle homogenates subject to SDS-PAGE, reacts relatively uniformly with a 43kD protein present in skeletal, cardiac, gizzard and aorta tissues. Appears to react with all isoforms of actin found in these preparations and shows a strong reaction with the alpha actin found in skeletal, cardiac and arterial muscle. Predicted molecular weight: 43 kDa. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user. |
| Concentration | 0.5 mg/ml |
| Purity | Protein A purified |
| Buffer | Preservative: 0.1% Sodium AzideConstituents: 40% Glycerol, 10mM PBS. pH 7.6 |
| Research Area | Extracellular Matrix |
| Restrictions | For research use only |
External Information: Actin antibody
| Google Scholar | Find more references for clone 2Q1055 on Google Scholar™ |
| EMBL Harvester | Search human, mouse or rat genome for antigen Actin (Bioinformatic Harvester (EMBL)) |
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