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Détail du produit pour Adeno-Associated Virus 2 antibody
Adeno-Associated Virus 2 antibody
Aperçu
| Antigène: | Adeno-Associated Virus 2 (intact particle) |
| Type anticorps: | Monoclonal |
| Application: | Immunofluorescence (IF), Immunoprecipitation (IP), Enzyme Immunoassay (EIA) |
| Hôte: | Mouse |
| Quantité: | |
| Prix: | 400,00 € (Plus frais de livraison et TVA) |
| Numéro de commande: | ABIN112130 |
| Disponibilité: | 3 to 10 days until shipment |
Description du produit: Adeno-Associated Virus 2 antibody
| Antigène | Adeno-Associated Virus 2 (intact particle) |
| Immunogène | Adeno-associated virus capsid proteins and virus particles |
| Type anticorps | Monoclonal |
| Format | Purified |
| Isotype | IgG3 »Anticorps secondaires correspondants |
| Clone | A20 |
| Hôte | Mouse |
| Specificité | For characterization of different stages of infection and very useful for the analysis of the AAV assembly process. A20 specifically reacts with intact adeno-associated virus particles, empty and full capsids. Recognizes a conformational epitope of assembled capsids, not present in denatured capsid proteins and native but unassembled capsid proteins. The antibody cannot be used for immunoblotting. Epitope mapping experiments (Wobus et al., see below) identified four immunoreactive (discontinous) regions. The major reaction was attributed to sequence aa 369 to aa378 of AAV-2 capsids. The antibody is also useful for neutralizing experiments (cf. Moskalenko et. al). Antibody reacts with AAV-2, found in human and monkey (species tested so far). In ELISA also applicable to AAV-3 |
Applications: Adeno-Associated Virus 2 antibody
| Application | Immunofluorescence (IF), Immunoprecipitation (IP), Enzyme Immunoassay (EIA) |
| Indications d'application | Immunofluorescence microscopy ( 1/20, overnight incubation at 2-8°C). Immunohistochemistry on frozen sections ( overnight incubation at 2-8°C) Immunoprecipitation (1/5). Affinity chromatography. ELISA. Neutralization assay. Other applications not tested. Optimal dilutions of this antibody are dependent on conditions and should be determined by the user. |
| Buffer | This antibody is a Protein A affinity purified immunoglobulin fraction and was lyophilised from PBS buffer with 0.09% sodium azide as preservative and 0.5% BSA as stabilizer Reconstitute with 1 ml distilled water. Dilute with PBS or Tris-buffered saline |
| Stock | Store at 2-8°C. Shelf life: one year after reconstitution. |
| Domaine de recherche | Virology |
| Restrictions | For Research Use only |
Références: Adeno-Associated Virus 2 antibody
| Références |
Moskalenko, Chen, van Roey et al.: "Epitope mapping of human anti-adeno-associated virus type 2 neutralizing antibodies: implications for gene therapy and virus structure." In: Journal of virology , Vol. 74, Issue 4, pp. 1761-6, 2000/01 (PubMed) Wistuba, Kern, Weger et al.: "Subcellular compartmentalization of adeno-associated virus type 2 assembly." In: Journal of virology , Vol. 71, Issue 2, pp. 1341-52, 1997/01 (PubMed) Wistuba, Weger, Kern et al.: "Intermediates of adeno-associated virus type 2 assembly: identification of soluble complexes containing Rep and Cap proteins." In: Journal of virology , Vol. 69, Issue 9, pp. 5311-9, 1995/01 (PubMed) Wobus, Huegle-Doerr, Girod et al.: "Monoclonal antibodies against the adeno-associated virus type 2 (AAV-2) capsid: epitope mapping and identification of capsid domains involved in AAV-2-cell interaction and neutralization of AAV-2 infection." In: Journal of virology , Vol. 74, Issue 19, pp. 9281-93, 2000/01 (PubMed) Grimm, Kern, Pawlita et al.: "Titration of AAV-2 particles via a novel capsid ELISA: packaging of genomes can limit production of recombinant AAV-2." In: Gene therapy , Vol. 6, Issue 7, pp. 1322-30, 2000/01 (PubMed) |
