Adiponectin (Human) antibody

Overview


	Antigen:	Adiponectin
	Antibody Type:	Polyclonal
	Reactivity:	Human
	Host:	Rabbit
	Quantity:	50 µg (Product variants)
	Price:	241,00 € (Plus shipping costs and VAT)
	Order number:	ABIN130495
	Availability:	7 to 10 days until shipment

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Additional Information: Adiponectin (Human) antibody

Antigen	Adiponectin
Immunogen	Polyclonal antibody is produced by immunizing rabbit with a recombinant murine adiponectin (GST-fusion protein) produced by E. coli. IgG is purified by immunoaffinity chromatography. Molecular Weight: 29 kDa
Reactivity	Human
Antibody Type	Polyclonal
Isotype	IgG »Matching secondary antibodies
Description	Adiponectin, also called GBP-28, apM1, AdipoQ and Acrp30, is a novel adipose tissue-specific protein that has structural homology to collagen VIII and X and complement factor C1q, and that circulates in human plasma at high levels. It is one of the physiologically active polypeptides secreted by adipose tissue, whose multiple functions have started to be understood in the last few Years. A reduction in adiponectin expression is associated with insulin resistance in some animal models. Administration of adiponectin has been accompanied by a reduction in plasma glucose and an increase in insulin sensitivity. In addition, thiazolidinediones, drugs that enhance insulin sensitivity through stimulation of the peroxisome proliferator-activated receptor-gamma, increase plasma adiponectin and mRNA levels in mice. On the other hand, this adipocyte protein seems to play a protective role in experimental models of vascular injury. In humans, adiponectin levels are inversely related to the degree of adiposity and positively associated with insulin sensitivity both in healthy subjects and in diabetic patients. Plasma adiponectin levels have been reported to be decreased in some insulin-resistant states, such as obesity and type 2 diabetes mellitus, and also in patients with coronary artery disease. On the contrary, chronic renal failure, type 1 diabetes and anorexia nervosa are associated with increased plasma adiponectin levels. Concentrations of plasma adiponectin have been shown to correlate negatively with glucose, insulin, triglyceride levels and body mass index, and positively with high-density lipoprotein-cholesterol levels and insulin-stimulated glucose disposal. Weight loss and therapy with thiazolidinediones increased endogenous adiponectin production in humans. Adiponectin increases insulin sensitivity by increasing tissue fat oxidation, resulting in reduced circulating fatty acid levels and reduced intracellular triglyceride contents in liver and muscle.
Host	Rabbit
Specificity	Mouse Adiponectin Antibody detects endogenous levels of adiponectin protein.

Application Details: Adiponectin (Human) antibody

Application Notes	Olutions and Reagents Note: Prepare solutions with Milli-Q or equivalently purified water. Transfer Buffer - 25 mM Tris base, 0.2 M glycine, 20% methanol (pH 8.5) SDS Sample Buffer (1X): 62.5 mM Tris-HCl (pH 6.8 at 25°C), 2% w/v SDS, 10% glycerol, 50 mM DTT, 0.01% w/v bromophenol blue or phenol red Blocking WB, IP Species Cross Reactivity H, M, R
Buffer	1X TBS, 0.1% Tween-20 with 5% w/v nonfat dry milk, for 150 mL, add 15 mL 10X TBS to 135 mL water, mix. Add 7.5 g nonfat dry milk and mix well. While stirring, add 0.15 mL Tween-20 (100%). 10X TBS (Tris-buffered saline): To prepare 1 liter of 10X TBS: 24.2
Restrictions	For Research Use Only

Publications: Adiponectin (Human) antibody

Publications

Combs, Wagner, Berger et al.:
"Induction of adipocyte complement-related protein of 30 kilodaltons by PPARgamma agonists: a potential mechanism of insulin sensitization."
In: Endocrinology , Vol. 143, Issue 3, pp. 998-1007, 2002/01 (PubMed)

Maeda, Takahashi, Funahashi et al.:
"PPARgamma ligands increase expression and plasma concentrations of adiponectin, an adipose-derived protein."
In: Diabetes , Vol. 50, Issue 9, pp. 2094-9, 2001/01 (PubMed)

Weyer, Funahashi, Tanaka et al.:
"Hypoadiponectinemia in obesity and type 2 diabetes: close association with insulin resistance and hyperinsulinemia."
In: The Journal of clinical endocrinology and metabolism , Vol. 86, Issue 5, pp. 1930-5, 2001/01 (PubMed)

Yamauchi, Kamon, Waki et al.:
"The fat-derived hormone adiponectin reverses insulin resistance associated with both lipoatrophy and obesity."
In: Nature medicine , Vol. 7, Issue 8, pp. 941-6, 2001/01 (PubMed)

Yang, Jeng, Wu et al.:
"Synthetic peroxisome proliferator-activated receptor-gamma agonist, rosiglitazone, increases plasma levels of adiponectin in type 2 diabetic patients."
In: Diabetes care , Vol. 25, Issue 2, pp. 376-80, 2002/01 (PubMed)

Images: Adiponectin (Human) antibody

Western blot analysis of Mouse Adiponectin

Image for Adiponectin (Human) antibody (1)

Product variants

Adiponectin (Human) antibody (Quantity: 50 µg)

External Information: Adiponectin (Human) antibody

Google Scholar	Find more references for antigen Adiponectin on Google Scholar™
EMBL Harvester	Search human, mouse or rat genome for antigen Adiponectin (Bioinformatic Harvester (EMBL))