Adipophilin (Human) antibody

Overview

Antigen:	Adipophilin (ADFP)
Antibody Type:	Polyclonal
Application:	Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Reactivity:	Human, Mouse (Murine), Rat
Host:	Guinea Pig
Quantity:	0.1 ml
Price:	461,03 $ (Plus shipping costs and VAT, Invoice in EUR)
Order number:	ABIN113458
Availability:	3 to 10 days until shipment

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Additional Information: Adipophilin (Human) antibody

Antigen	Adipophilin (ADFP)
Immunogen	Synthetic peptide (N-terminal aa 1-25 of murine adipophilin)
Reactivity	Human, Mouse (Murine), Rat
Antibody Type	Polyclonal
Format	Serum
Host	Guinea Pig
Specificity	New specific marker for liposarcoma. Adipophilin is present in milk fat globule membranes and on the surface of lipid droplets in various cultured cell lines, inducible by etomoxir*. Polypeptide reacting: Adipophilin, MW 48,100 (calculated from aa sequence data), apparent Mr 52,000 (after SDS-PAGE), pI 6.72. Immunolocalization: Adipophilin is positively detected in the glandular cells of lactating mammary gland (ductal cells are negative), zona fasciculata of the adrenal gland, Sertoli cells of the testis, and in fat-accumulating hepatocytes of alcoholic cirrhotic fatty liver, adipocytes are negative. Specific detection of liposarcoma. Also positively stained are lipid-storing CD 68-positive macrophages. Antigen recognized in species (tested so far): Human, mouse, rat, dog, bovine. Reactivities on Cultured Cell Lines (tested so far): Caco, PLC, HaCat, SV80, RD 125, Huvec (human umbilical cord endothelia), RV, PC-12 (rat adrenal gland), MDCK, negative with glioma.

Application Details: Adipophilin (Human) antibody

Application	Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Application Notes	Immunohistochemistry on frozen and paraffin sections: 1/100 (paraffin sections require heat mediated antigen retrieval by microwave treatment). Cytological material. Western blot: 1/2000 (ECL). Incubation time: 1h at RT or over night at 2-8°C. Other applications not tested. Optimal dilutions of this antibody are dependent on conditions and should be determined by the user.
Buffer	This antibody is supplied as whole antiserum with 0.09% sodium azide as preservative.
Storage	Store the antibody at 2-8°C. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Research Area	Metabolism
Restrictions	For Research Use only

Publications: Adipophilin (Human) antibody

Publications

Heid, Moll, Schwetlick et al.:
"Adipophilin is a specific marker of lipid accumulation in diverse cell types and diseases."
In: Cell and tissue research , Vol. 294, Issue 2, pp. 309-21, 1998/01 (PubMed)

Heid, Schnoelzer, Keenan:
"Adipocyte differentiation-related protein is secreted into milk as a constituent of milk lipid globule membrane."
In: The Biochemical journal , Vol. 320 ( Pt 3), pp. 1025-30, 1997/01 (PubMed)

Hope, McLauchlan:
"Sequence motifs required for lipid droplet association and protein stability are unique to the hepatitis C virus core protein."
In: The Journal of general virology , Vol. 81, Issue Pt 8, pp. 1913-25, 2000/01 (PubMed)

Steiner, Wahl, Mangold et al.:
"Induction of the adipose differentiation-related protein in liver of etomoxir-treated rats."
In: Biochemical and biophysical research communications , Vol. 218, Issue 3, pp. 777-82, 1996/01 (PubMed)

External Information: Adipophilin (Human) antibody

Google Scholar	Find more references for antigen Adipophilin (ADFP) on Google Scholar™
EMBL Harvester	Search human, mouse or rat genome for antigen Adipophilin (ADFP) (Bioinformatic Harvester (EMBL))