Aggrus/Podoplanin (Human) antibody

Overview


	Antigen:	Aggrus/Podoplanin (Human)
	Antibody Type:	Monoclonal
	Application:	Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Flow Cytometry (FACS)
	Host:	Rat, Mouse
	Quantity:	100 µg
	Price:	383,23 $ (Plus shipping costs , $13.07 dry ice fee and VAT, Invoice in EUR)
	Order number:	ABIN130715
	Availability:	7 to 10 days until shipment

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Additional Information: Aggrus/Podoplanin (Human) antibody

Antigen	Aggrus/Podoplanin (Human)
Immunogen	This antibody was concentrated from hybridoma (clone YM-1) supernatant. This hybridoma was established by fusion of mouse myeloma cell P3-U1 with Sprague-Dawley rat splenocyte immunized with human Aggrus N-terminal peptide (38a.a.-51a.a.)
Antibody Type	Monoclonal
Isotype	IgG2a »Matching secondary antibodies
Description	In addition to hemostasis and host defense, platelets are involved in the induction of inflammation, tissue repair, and tumor metastasis. Aggrus (T1a/Podoplanin) is a novel 36-45 kDa membrane sialoglycoprotein that induces platelet aggregation. Aggrus is highly expressed in various tumor cells and the platelet aggregation ability of Aggrus depends on specific oligosaccharide structures that may be related to the metastasis process in cancer cells. Aggrus has been identified as a diagnostic tumor marker for seminoma.
Clone	YM-1
Host	Rat, Mouse
Specificity	This antibody reacts with human Aggrus (36 kDa) on Western blotting.

Application Details: Aggrus/Podoplanin (Human) antibody

Application	Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Flow Cytometry (FACS)
Application Notes	Western blotting : 1:500 for chemiluminescence detection system. Immunoprecipitation: Not determined. Immunohistochemistry : 1:20. Immunocytochemistry : Not tested. Flow Cytometry : 1:25
Buffer	100 µL aliquot of concentrate antibody from the supernatant with preservative (0.09% sodium azide). *Azide may react with copper or lead in plumbing system to form explosive metal azides. Therefore, always flush drains thoroughly with plenty of water when
Restrictions	For Research Use Only

Publications: Aggrus/Podoplanin (Human) antibody

Publications

Kaneko, Kato, Kunita et al.:
"Functional sialylated O-glycan to platelet aggregation on Aggrus (T1alpha/Podoplanin) molecules expressed in Chinese hamster ovary cells."
In: The Journal of biological chemistry , Vol. 279, Issue 37, pp. 38838-43, 2004/01 (PubMed)

Kato, Fujita, Kunita et al.:
"Molecular identification of Aggrus/T1alpha as a platelet aggregation-inducing factor expressed in colorectal tumors."
In: The Journal of biological chemistry , Vol. 278, Issue 51, pp. 51599-605, 2003/01 (PubMed)

Kato, Sasagawa, Kaneko et al.:
"Aggrus: a diagnostic marker that distinguishes seminoma from embryonal carcinoma in testicular germ cell tumors."
In: Oncogene , Vol. 23, Issue 52, pp. 8552-6, 2004/01 (PubMed)

Images: Aggrus/Podoplanin (Human) antibody

Immunohistochemical detection of Human Aggrus/Podoplanin on paraffin embedded section of Human normal rectum with ABIN130715.

Image for Aggrus/Podoplanin (Human) antibody (1)

Flow cytometric analysis of Human Aggrus/Podoplanin expression on LEC . Open histogram indicates the reaction of Isotypic control to LEC . Shaded histogram indicates the reaction of ABIN130715 to LEC.

Image for Aggrus/Podoplanin (Human) antibody (2)

Western blot analysis of Human Aggrus/Podoplanin expression in LEC (1) and mock transfected CHO cells (2) using ABIN130715.

Image for Aggrus/Podoplanin (Human) antibody (3)

External Information: Aggrus/Podoplanin (Human) antibody

Google Scholar	Find more references for clone YM-1 on Google Scholar™
EMBL Harvester	Search human, mouse or rat genome for antigen Aggrus/Podoplanin (Human) (Bioinformatic Harvester (EMBL))