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Product details for C3c (Human) antibody
C3c (Human) antibody
Overview
| Antigen: | C3c |
| Antibody Type: | Polyclonal |
| Application: | Immunoelectrophoresis (IEP), Radioimmunoassay (RIA), Rocket Immunoelectrophoresis (IEP) |
| Reactivity: | Human, Mouse (Murine), Rat, Dog (Canine) |
| Host: | Sheep |
| Quantity: | |
| Price: | 271,00 € (Plus shipping costs and VAT) |
| Order number: | ABIN150640 |
| Availability: | 7 to 10 days until shipment |
Additional Information: C3c (Human) antibody
| Antigen | C3c |
| Immunogen | Human C3c purified from serum and shown to be pure by SDS-PAGE. |
| Reactivity | Human, Mouse (Murine), Rat, Dog (Canine) |
| Antibody Type | Polyclonal |
| Isotype | IgG »Matching secondary antibodies |
| Description | C3 consists of two polypeptide chains (alpha, molecular weight 115 kDa and beta, molecular weight 75 kDa) and is usually present in serum at 1000-2000 mg/L. It is the pivotal protein of the complement system, being central in the classical pathway and the alternative pathway. It is cleaved by the classical pathway convertase C4b2a and the alternative pathway convertase C3bBb, into C3b and C3a. The C3a peptide is an important anaphylotoxin, whilst the C3b has three main roles: 1. Important opsonin-phagocyte cells have C3b receptors. 2. Participates in the amplification loop in the alternative pathway. 3. Complexed with C4b2a this is the C5 convertase, which leads ultimately to target cell lysis. C3c, along with iC3b and C3dg, is formed as a result of C3b inactivation by Factor I and various cofactors. The C3 fragments are eliminated by liver phagocytic cells. |
| Host | Sheep |
| Specificity | This product gives a single arc when tested by IEP and 2D-IEP against fresh human plasma.Identity has been confirmed by double diffusion (Ouchterlony) vs fresh human plasma and a known anti-human C3c. |
Application Details: C3c (Human) antibody
| Application | Immunoelectrophoresis (IEP), Radioimmunoassay (RIA), Rocket Immunoelectrophoresis (IEP) |
| Application Notes | RID and Rocket IEP: 1uL antiserum/cm?? gel vs 5uL 1/5-1/50 dilution human plasmaDouble diffusion: 10uL antiserum vs 5uL plasmaIEP: 100uL antiserum vs 5uL plasmaThe use of 3% PEG 6000 with 1.2%agarose in a suitable buffer (such as TBE or Tris-barbital pH >8.2) is recommended. Suitability for use in immunohistochemical procedures, nephelometry, enzyme-linked immunosorbent assays and Western blot has not been assessed butuse in such assays should not necessarily be excluded. |
| Concentration | Batch Dependent |
| Purity | IgG fraction |
| Buffer | Glycine buffered saline pH7.4, 0.1% Sodium Azide, 0.1% EACA, 0.01% Benzamidine,1mM EDTA |
| Storage | Store at 4 C where it will remain stable until the given expiry date. Slight precipitation can occur on storage which may be removed by centrifugation and should not affectperformance characteristics. |
| Restrictions | For research use only |
External Information: C3c (Human) antibody
| Google Scholar | Find more references for antigen C3c on Google Scholar™ |
| EMBL Harvester | Search human, mouse or rat genome for antigen C3c (Bioinformatic Harvester (EMBL)) |
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