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Product details for CD11a Mouse (Murine) antibody
CD11a Mouse (Murine) antibody
Overview
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| Antigen: | CD11a |
| Antibody Type: | Monoclonal |
| Application: | Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Blocking Antibody (BLOCK) |
| Conjugate: | LEAF |
| Reactivity: | Mouse (Murine) |
| Host: | Rat |
| Quantity: | |
| Price: | 81,00 € (Plus shipping costs and VAT) |
| Order number: | ABIN155486 |
| Availability: | Ships within 7 to 10 days |
Additional Information: CD11a Mouse (Murine) antibody
| Antigen | CD11a |
| Immunogen | C57BL/6 mouse splenic secondary cytotoxic T cells |
| Reactivity | Mouse (Murine) |
| Antibody Type | Monoclonal |
| Format | Sterile Low-Endotoxin, Azide-Free formulation (LEAF) |
| Isotype | Rat IgG2a, kappa »Matching secondary antibodies |
| Description | CD11a is a 180 kD glycoprotein, also known as alphaL integrin, LFA-1 alpha, Ly-15, or Ly-21. It is a member of the integrin family, primarily expressed on lymphocytes, monocytes/macrophages, and granulocytes. In association with CD18, the CD11a/CD18 complex forms LFA-1. CD11a plays an important role in intercellular adhesion and costimulation by binding its ligands, ICAM-1 (CD54), ICAM-2 (CD102), and ICAM-3 (CD50). The M17/4 antibody can block CD11a-mediated cellular adhesion. Distribution: Lymphocytes, granulocytes, monocytes, macrophages Function: Cellular adhesion Synonyms: alphaL integrin, LFA-1 alpha subunit, Ly-15, Ly-21 Structure: Integrin family, associates with integrin beta2 (CD18), 180 kD |
| Clone | M17-4 |
| Host | Rat |
Application Details: CD11a Mouse (Murine) antibody
| Application | Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Blocking Antibody (BLOCK) |
| Application Notes | Additional reported applications of this antibody (for the relevant formats) include: immunoprecipitation, in vitro blocking of cell-cell adhesion, and immunohistochemical staining of acetone-fixed frozen sections. The M17/4 antibody does not block the binding of 2D7 antibody (ABIN155471) to CD11a. The LEAF(TM) purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm sterile-filtered) is recommended for functional assays (ABIN155487). Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is < 0.25 µg per 10 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. |
| Conjugate | LEAF |
| Purification | The antibody was purified by affinity chromatography. |
| Buffer | Phosphate-buffered solution, pH 7.2, containing no preservative. 0.2 µm filter sterilized. Endotoxin level is < 0.1 EU/µg of the protein (< 0.01 ng/µg of the protein) as determined by the LAL test. |
| Storage | The antibody solution should be stored undiluted at 4 °C. This LEAF(TM) solution contains no preservative, handle under aseptic conditions. |
| Research Area | CD Antigens |
| Restrictions | For Research Use only |
Publications: CD11a Mouse (Murine) antibody
| Publications |
Lub, van Kooyk, Figdor: "Ins and outs of LFA-1." In: Immunology today , Vol. 16, Issue 10, pp. 479-83, 1995/01 (PubMed) Springer: "Traffic signals for lymphocyte recirculation and leukocyte emigration: the multistep paradigm." In: Cell , Vol. 76, Issue 2, pp. 301-14, 1994/01 (PubMed) Hailman, Allen: "Inefficient cell spreading and cytoskeletal polarization by CD4+CD8+ thymocytes: regulation by the thymic environment." In: Journal of immunology (Baltimore, Md. : 1950) , Vol. 175, Issue 8, pp. 4847-57, 2005/01 (PubMed) Kuhlman, Moy, Lollo et al.: "The accessory function of murine intercellular adhesion molecule-1 in T lymphocyte activation. Contributions of adhesion and co-activation." In: Journal of immunology (Baltimore, Md. : 1950) , Vol. 146, Issue 6, pp. 1773-82, 1991/01 (PubMed) Mizgerd, Kubo, Kutkoski et al.: "Neutrophil emigration in the skin, lungs, and peritoneum: different requirements for CD11/CD18 revealed by CD18-deficient mice." In: The Journal of experimental medicine , Vol. 186, Issue 8, pp. 1357-64, 1997/01 (PubMed) Sanchez-Madrid, Davignon, Martz et al.: "Antigens involved in mouse cytolytic T-lymphocyte (CTL)-mediated killing: functional screening and topographic relationship." In: Cellular immunology , Vol. 73, Issue 1, pp. 1-11, 1983/01 (PubMed) |
Images: CD11a Mouse (Murine) antibody
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C57BL/6 mouse splenocytes stained with LEAF(TM) purified M17/4, followed by anti-rat IgGs FITC |
Product variants
External Information: CD11a Mouse (Murine) antibody
| Google Scholar | Find more references for clone M17-4 on Google Scholar™ |
| EMBL Harvester | Search human, mouse or rat genome for antigen CD11a (Bioinformatic Harvester (EMBL)) |
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