Product details for  CD11b (activated) Human antibody
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CD11b (activated) Human antibody

Overview

Antigen: CD11b (activated)
Antibody Type: Monoclonal
Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Blocking Antibody (BLOCK)
Conjugate: LEAF
Reactivity: Human
Host: Mouse
Quantity: 500 µg (Product variants)
Price: 405,00 €   (Plus shipping costs and VAT)
Order number: ABIN157664
Availability: Ships within 7 to 10 days
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Additional Information:  CD11b (activated) Human antibody

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Antigen CD11b (activated)
Reactivity Human
Antibody Type Monoclonal
Format Sterile Low-Endotoxin, Azide-Free formulation (LEAF)
Isotype Mouse IgG1, kappa »Matching secondary antibodies
Description The CBRM1/5 antibody reacts with an activated form of human CD11b, a 165-170 kD type I transmembrane glycoprotein also known as alphaM integrin, Mac-1, CR3, and C3biR. CD11b non-covalently associates with integrin beta2 (CD18) and is expressed on granulocytes, monocytes/macrophages dendritic cells, NK cells, and subsets of T and B cells. CD11b/CD18 is critical for the transendothelial migration of monocytes and neutrophils, as well as adhesion, phagocytosis and neutrophil activation. CD11b/CD18 interacts with ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4, CD14, CD23, heparin, iC3b, fibrinogen and Factor X. The CBRM1/5 antibody recognizes a subset of CD11b molecules on neutrophils and monocytes activated with chemoattractants or phorbol-esters. This antibody does not recognize non-activated CD11b. The epitope recognized by CBRM1/5 is contained in the I domain of the alpha chain. The CBRM1/5 antibody blocks cell adhesion to fibrinogen and ICAM-1. Distribution: Granulocytes, monocytes/macrophages, dendritic cells, NK cells, T cell subset, B cell subset Function: Adhesion, phagocytosis, chemotaxis, neutrophil activation Synonyms: Integrin alphaM chain, C3biR, CR3, Mac-1, Mo1 Structure: Integrin, type I transmembrane glycoprotein, associates with integrin beta2 (CD18), 165-170 KD
Clone CBRM1-5
Host Mouse

Application Details:  CD11b (activated) Human antibody

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Application Flow Cytometry (FACS), Immunoprecipitation (IP), Blocking Antibody (BLOCK)
Application Notes Additional reported applications (for the relevant formats) include: immunoprecipitation, and blocking of the binding of ICAM-1 and fibrinogen. The CBRM1/5 antibody binds specifically to the activated form of CD11b. The LEAF(TM) purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm sterile-filtered) is recommended for functional assays (ABIN157664). Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, it is recommended to use at < 1.0 µg per 10 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Conjugate LEAF
Purification The antibody was purified by affinity chromatography.
Buffer Phosphate-buffered solution, pH 7.2, containing no preservative. 0.2 µm filter sterilized. Endotoxin level is < 0.1 EU/µg of the protein (< 0.01 ng/µg of the protein) as determined by the LAL test.
Storage The antibody solution should be stored undiluted at 4 °C. This LEAF(TM) solution contains no preservative, handle under aseptic conditions.
Research Area CD Antigens
Restrictions For Research Use only

Publications:  CD11b (activated) Human antibody

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Publications Petty, Todd:
"Integrins as promiscuous signal transduction devices."
In: Immunology today , Vol. 17, Issue 5, pp. 209-12, 1997/01 (PubMed)

Stewart, Thiel, Hogg:
"Leukocyte integrins."
In: Current opinion in cell biology , Vol. 7, Issue 5, pp. 690-6, 1996/01 (PubMed)

Bryn, Mahic, Enserink et al.:
"The cyclic AMP-Epac1-Rap1 pathway is dissociated from regulation of effector functions in monocytes but acquires immunoregulatory function in mature macrophages."
In: Journal of immunology (Baltimore, Md. : 1950) , Vol. 176, Issue 12, pp. 7361-70, 2006/01 (PubMed)

Diamond, Springer:
"A subpopulation of Mac-1 (CD11b/CD18) molecules mediates neutrophil adhesion to ICAM-1 and fibrinogen."
In: The Journal of cell biology , Vol. 120, Issue 2, pp. 545-56, 1993/01 (PubMed)

Sithu, English, Olson et al.:
"Membrane-type 1-matrix metalloproteinase regulates intracellular adhesion molecule-1 (ICAM-1)-mediated monocyte transmigration."
In: The Journal of biological chemistry , Vol. 282, Issue 34, pp. 25010-9, 2007/01 (PubMed)

External Information:  CD11b (activated) Human antibody

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Google Scholar Find more references for clone CBRM1-5 on Google Scholar™
EMBL Harvester Search human, mouse or rat genome for antigen CD11b (activated) (Bioinformatic Harvester (EMBL))