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Product details for CD140b Human antibody
CD140b Human antibody
Overview
| Antigen: | CD140b |
| Antibody Type: | Monoclonal |
| Application: | Flow Cytometry (FACS) |
| Conjugate: | APC |
| Reactivity: | Human |
| Host: | Mouse |
| Quantity: | |
| Price: | 145,00 € (Plus shipping costs and VAT) |
| Order number: | ABIN179931 |
| Availability: | Ships within 7 to 10 days |
Additional Information: CD140b Human antibody
| Antigen | CD140b |
| Immunogen | NIH-3T3 cells transfected with human PDGFRbeta |
| Reactivity | Human |
| Antibody Type | Monoclonal |
| Isotype | Mouse IgG1 Mouse IgG1, kappa »Matching secondary antibodies |
| Description | The 18A2 monclonal antibody recognizes human CD140b also known as the platelet-derived growth factor receptor, beta polypeptide, PDGFR1, and PDGFRbetaï€ CD140b is a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. The identity of the growth factor bound to the receptor determines whether the functional receptor is a homodimer or heterodimer composed of both PDGFR-alpha and -beta. CD140b contains two immunoglobulin-like domains and a tyrosine kinase domain with a predicted molecular weight approximately 124 kD.ï€ CD140b is widely expressed on a variety of mesenchymal-derived cells and is preferentially expressed on some tumors such as medulloblastoma. Binding of B-chain containing PDGF molecules can stimulate cell proliferation. CD140b has been shown to interact with a number of kinases (including Raf-1, NCK1, FAK, Fyn, others) as well as adaptor molecules and signaling intermediates (Crk, Grb2, Grb4, RasGAP, SHP2, SHC1, others), and has also been shown to associate with integrin beta3 and nexin sorting molecules. CD140b has been implicated in several disease states including atherogenesis and oncogenesis. The PDGFRbeta is heavily phosphorylated on numerous tyrosine residues through both autophosphorylation and ligand-dependent processes. The 18A2 antibody has been shown to be useful for flow cytometric detection of CD140b. Distribution: Widely expressed on a variety of mesenchymal-derived cells. Preferentially expressed in medulloblastoma. Function: Stimulation of cell proliferation, mitogenic activity for cells of mesenchymal origin. Has been implicated in atherogenesis and oncogenesis. Modifications: Multiple tyrosine phosphorylation sites (Y549, Y581, Y716, Y740, Y751, Y763, Y771, Y775, Y857, Y1009, Y1021) Synonyms: Platelet-derived growth factor receptor, beta polypeptide, PDGFR1, PDGFRbeta, PDGFRb, PDGF receptor beta Structure: Cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. The identity of the growth factor bound to the receptor determines whether the functional receptor is a homodimer or heterodimer composed of both PDGFR1 and PDGFR2. Contains two immunoglobulin-like domains and a tyrosine kinase domain, predicted molecular weight approximately 124 kD. |
| Clone | 18A2 |
| Host | Mouse |
| Specificity | Interacts with a number of kinases (including Raf-1, NCK1, FAK, Fyn, others) as well as adaptor molecules and signaling intermediates (Grb2, Grb4, RasGAP, SHP2, SHC1, Crk, others). Has also been shown to associate with integrin beta3 and nexin sorting molecules. |
Application Details: CD140b Human antibody
| Application | Flow Cytometry (FACS) |
| Application Notes | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is 20 µl per 1 million cells or 20 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. |
| Conjugate | APC |
| Purification | The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions. The solution is free of unconjugated APC and unconjugated antibody. |
| Buffer | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA). |
| Storage | The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. |
| Research Area | CD Antigens |
| Restrictions | For Research Use only |
External Information: CD140b Human antibody
| Google Scholar | Find more references for clone 18A2 on Google Scholar™ |
| EMBL Harvester | Search human, mouse or rat genome for antigen CD140b (Bioinformatic Harvester (EMBL)) |
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