Product details for  CD1-PE (Porcine) antibody
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CD1-PE (Porcine) antibody

Overview

Antigen: CD1
Antibody Type: Monoclonal
Application: Flow Cytometry (FACS)
Conjugate: PE
Reactivity: Pig (Porcine)
Host: Mouse
Quantity: 0,1 mg
Price: 221,00 €   (Plus shipping costs and VAT)
Order number: ABIN135463
Availability: 7 to 10 days until shipment
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Additional Information:  CD1-PE (Porcine) antibody

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Antigen CD1
Reactivity Pig (Porcine)
Antibody Type Monoclonal
Isotype IgG2a »Matching secondary antibodies
Description Porcine CD1, Mr 43-49 kDa Porcine CD1 is a type I transmembrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It has a domain organization similar to that of MHC class I molecules and is expressed in association with 2-microglobulin. CD1 is found on B cells, macrophages and immature thymocytes. There is evidence for a role of CD1 in presentation of lipids and peptides to T cells.
Clone 76-7-4
Host Mouse

Application Details:  CD1-PE (Porcine) antibody

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Application Flow Cytometry (FACS)
Application Notes Working Dilutions: Flow Cytometry: Fluorescein (1 ug/10^6 cells), Biotin (1 ug/10^6 cells), R-phycoerythrin (0.2 ug/10^6 cells). Since applications vary, you should determine the optimum working dilution of the product that is appropriate for your specific need.
Conjugate PE
Storage The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.0. No preservatives or amine-containing buffer salts added. Store at 2-8C. The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8C. The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8C. The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8C. Do not freeze! Protect conjugated forms from light. Reagents are stable for the period shown on the label if stored as directed. WARNING Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Research Area CD Antigens, Surface Receptors of Immune Cells
Restrictions For Research Use only

Publications:  CD1-PE (Porcine) antibody

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Publications Denham, Shimizu, Bianchi et al.:
"Monoclonal antibodies recognising differentiation antigens on porcine B cells."
In: Veterinary immunology and immunopathology , Vol. 43, Issue 1-3, pp. 259-67, 1995/01 (PubMed)

Pescovitz, Lunney, Sachs:
"Preparation and characterization of monoclonal antibodies reactive with porcine PBL."
In: Journal of immunology (Baltimore, Md. : 1950) , Vol. 133, Issue 1, pp. 368-75, 1984/01 (PubMed)

External Information:  CD1-PE (Porcine) antibody

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Google Scholar Find more references for clone 76-7-4 on Google Scholar™
EMBL Harvester Search human, mouse or rat genome for antigen CD1 (Bioinformatic Harvester (EMBL))