CD3 (Human) antibody

Overview


	Antigen:	CD3
	Antibody Type:	Polyclonal
	Application:	Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Western Blotting (WB)
	Reactivity:	Mouse (Murine), Human, Chicken, Rat
	Host:	Rabbit
	Quantity:	50 (0.5 mg/ml) (Product variants)
	Price:	200,00 € (Plus shipping costs and VAT)
	Order number:	ABIN123245
	Availability:	Ships in 3 to 10 days

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Additional Information: CD3 (Human) antibody

Antigen	CD3
Immunogen	Rabbit anti-CD3 polyclonal antibody was raised against the synthetic peptide corresponding to amino acids 156- 168 of human CD3 protein, Epsilon chain, only one amino acid different from mouse and rat origins. Human tonsil stained with anti- CD3 antibody ABIN123245 by IHC
Reactivity	Mouse (Murine), Human, Chicken, Rat
Antibody Type	Polyclonal
Format	Aff - Purified
Host	Rabbit
Specificity	T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3., CD3d, CD3e and CD3. (1, 2). These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11 (1). The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3e may play a role in TCR-induced growth arrest, cell survival and proliferation (3). This epitope affinity purified rabbit polyclonal antibody is specific for CD3.

Application Details: CD3 (Human) antibody

Application	Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Western Blotting (WB)
Application Notes	ELISA: 0.1-1.0 µg/ml WB: 0.5 - 2 µg/ml IHC: 1:100 for 10 min at RT By IHC on human tonsil, a postive cell membrane staining is observed in Formalin-fixed tissues (boiling in 10 mM citrate buffer, pH 6.0 for 10 min, followed by cooling at RT for 20 min). Other applications not tested. Optimal dilutions of this antibody are dependent on conditions and should be determined by the user: The results might be affected by several factors, including secondary antibody affinity, antigen concentration, sensitivity of detection method, temperature and length of incubations.
Concentration	0.5 mg/ml
Buffer	This polyclonal antibody is supplied as an epitope affinity purified rabbit IgG, 100 µg in 200 µl of phosphate buffered saline (pH 7.4) containing 0.02% sodium azide.
Storage	Store at 2-8 oC for up to one month. Store at –20 oC for long-term storage. Avoid repeated freezing and thawing. Shelf life: one year from despatch
Research Area	CD Antigens, Surface Receptors of Immune Cells
Restrictions	Research only

Publications: CD3 (Human) antibody

Publications

Malissen, Schmitt-Verhulst:
"Transmembrane signalling through the T-cell-receptor-CD3 complex."
In: Current opinion in immunology , Vol. 5, Issue 3, pp. 324-33, 1993/01 (PubMed)

Gil, Gutierrez, Alarcon:
"Intracellular redistribution of nucleolin upon interaction with the CD3epsilon chain of the T cell receptor complex."
In: The Journal of biological chemistry , Vol. 276, Issue 14, pp. 11174-9, 2001/01 (PubMed)

Clevers, Alarcon, Wileman et al.:
"The T cell receptor/CD3 complex: a dynamic protein ensemble."
In: Annual review of immunology , Vol. 6, pp. 629-62, 1988/01 (PubMed)

Images: CD3 (Human) antibody

Human tonsil stained with anti-CD3 antibody ABIN123245 by IHC

Image for CD3 (Human) antibody (1)

Product variants

CD3 (Human) antibody (Format: Purified, Quantity: 1 ml)

CD3 (Human) antibody (Format: Purified, Quantity: 0,5 ml)

CD3 (Human) antibody (Format: Purified, Quantity: 0.5 ml)

External Information: CD3 (Human) antibody

Google Scholar	Find more references for antigen CD3 on Google Scholar™
EMBL Harvester	Search human, mouse or rat genome for antigen CD3 (Bioinformatic Harvester (EMBL))