TNFRSF8 antibody (FITC)
(1 reference)Quick Overview for TNFRSF8 antibody (FITC) (ABIN192353)
Target
See all TNFRSF8 AntibodiesReactivity
Host
Clonality
Conjugate
Application
Clone
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Purpose
- Anti-Hu CD30 FITC
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Cross-Reactivity (Details)
- Human
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Characteristics
- The antibody MEM-268 recognizes extracellular part of CD30 (Ki-1 antigen), a 105 kDa single chain glycoprotein expressed on Hodgkin's and Reed-Sternberg cells, it is also found in Burkitt's lymphomas, virus-infected T and B lymphocytes, and on normal B and T lymphocytes after activation (T lymphocytes that produce Th2-type cytokines and on CD4+/CD8+ T lymphocytes that co-express CD45RO and the IL4 receptor).
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Purification
- Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
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Immunogen
- Expression vector containing CD30 cDNA (booster suspension of THP-1 cell line)
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Isotype
- IgG2a, kappa
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anti-Tumor Necrosis Factor Receptor Superfamily, Member 8 (TNFRSF8) (AA 19-379) antibody
TNFRSF8 Reactivity: Human IHC, WB, IHC (p) Host: Mouse Monoclonal 1C6 unconjugated
anti-Tumor Necrosis Factor Receptor Superfamily, Member 8 (TNFRSF8) (AA 19-379) antibodyTNFRSF8 Reactivity: Human IHC, WB, IHC (p) Host: Mouse Monoclonal 18D3 unconjugated
anti-Tumor Necrosis Factor Receptor Superfamily, Member 8 (TNFRSF8) antibodyTNFRSF8 Reactivity: Human FACS, WB, ELISA Host: Mouse Monoclonal 3B10 unconjugated
anti-Tumor Necrosis Factor Receptor Superfamily, Member 8 (TNFRSF8) antibodyTNFRSF8 Reactivity: Human, Mouse, Rat IHC, IF Host: Rabbit Polyclonal unconjugated
anti-Tumor Necrosis Factor Receptor Superfamily, Member 8 (TNFRSF8) (AA 19-379) antibodyTNFRSF8 Reactivity: Human FACS, ELISA Host: Mouse Monoclonal 4D1C2 unconjugated
anti-Tumor Necrosis Factor Receptor Superfamily, Member 8 (TNFRSF8) antibody (PE)TNFRSF8 Reactivity: Human FACS Host: Mouse Monoclonal MEM-268 PE
anti-Tumor Necrosis Factor Receptor Superfamily, Member 8 (TNFRSF8) antibodyTNFRSF8 Reactivity: Human FACS, IHC (p) Host: Mouse Monoclonal MEM-268 unconjugated
anti-Tumor Necrosis Factor Receptor Superfamily, Member 8 (TNFRSF8) (AA 131-230) antibodyTNFRSF8 Reactivity: Human, Pig FACS, WB, ELISA, IHC (p), IF (p), IF (cc), IHC (fro) Host: Rabbit Polyclonal unconjugated
anti-Tumor Necrosis Factor Receptor Superfamily, Member 8 (TNFRSF8) antibody (PE)TNFRSF8 Reactivity: Human FACS Host: Mouse Monoclonal Ber-H8 PE
anti-Tumor Necrosis Factor Receptor Superfamily, Member 8 (TNFRSF8) antibody (APC)TNFRSF8 Reactivity: Human FACS Host: Mouse Monoclonal MEM-268 APC
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Application Notes
- Flow cytometry: The reagent is designed for analysis of human blood cells using 20 μL reagent / 100 μL of whole blood or 106 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.
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Restrictions
- For Research Use only
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Format
- Liquid
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Buffer
- Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
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Preservative
- Sodium azide
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Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Handling Advice
- Do not freeze,Protect from prolonged exposure to light
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Storage
- 4 °C
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Storage Comment
- Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
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: "Development and validation of a fluorescent microsphere immunoassay for soluble CD30 testing." in: Clinical and vaccine immunology : CVI, Vol. 16, Issue 9, pp. 1327-31, (2009) (PubMed).
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- TNFRSF8 (Tumor Necrosis Factor Receptor Superfamily, Member 8 (TNFRSF8))
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Alternative Name
- CD30
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Background
- TNF receptor superfamily member 8,CD30 is a type I transmembrane glycoprotein of the TNF receptor superfamily. CD30 was originally identified as a cell surface antigen of Hodgkins and Reed-Sternberg cells using monoclonal antibody Ki-1. The ligand for CD30 is CD30L (CD153). The binding of CD30 to CD30L mediates pleiotropic effects including cell proliferation, activation, differentiation, and apoptotic cell death. CD30 has a critical role in the pathophysiology of Hodgkin's disease and other CD30+ lymphomas. CD30 acts as a costimulatory molecule in thymic negative selection. In addition to its expression on Hodgkin's and Reed-Sternberg cells, CD30 is also found in some non-Hodgkin's lymphomas (including Burkitt's lymphomas), virus-infected T and B cells, and on normal T and B cells after activation. In T cells, CD30 expression is present on a subset of T cells that produce Th2-type cytokines and on CD4+/CD8+ thymocytes that co-express CD45RO and the IL4 receptor. Soluble form of CD30 (sCD30) serves as a marker reflecting Th2 immune response.,Ki-1, TNFRSF8, CD30L receptor, D1S166E
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Gene ID
- 943
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UniProt
- P28908
Target
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