Product details for  CD45.1/Ly-5.1-FITC (Mouse) antibody
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CD45.1/Ly-5.1-FITC (Mouse) antibody

Overview

Antigen: CD45.1/Ly-5.1
Antibody Type: Monoclonal
Application: Flow Cytometry (FACS)
Conjugate: FITC
Reactivity: Mouse (Murine)
Host: Mouse
Quantity: 0,5 mg
Price: 369,00 €   (Plus shipping costs and VAT)
Order number: ABIN135321
Availability: 7 to 10 days until shipment
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Additional Information:  CD45.1/Ly-5.1-FITC (Mouse) antibody

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Antigen CD45.1/Ly-5.1
Reactivity Mouse (Murine)
Antibody Type Monoclonal
Isotype IgG2a »Matching secondary antibodies
Description Mouse CD 45.1 (Leukocyte Common Antigen) The CD45 antigen is an essential regulator of leukocyte activation and development, and is now known to be a protein tyrosine phosphatase, e.g. CD45 is able to dephosphorylate the Src family of protein kinases.1, 2 This activity is now known to be required for signal transduction induced by T and B cell antigen receptor engagement, cytokine signaling and Fc receptor stimulation.1, 2 The functional state of CD45 also has a pronounced effect on lymphocyte development. CD45 exists in multiple forms, such as the restricted variants (CD45R, see companion products 1660, 1665, 1675, 1685 and 1820) and as allelic forms CD45.1/Ly-5.1 and CD45.2/Ly-5.2. The former arises as a result of alternative splicing of different exons. The specificity of antibodies to CD45.1 and CD45.2 has been exploited in studies where resolution of donor and recipient cells is essential, such as in gene therapy or bone marrow transplantation.3, 4 The monoclonal antibody A20 recognizes all mouse leukocytes expressing the CD 45.1 allotype.
Clone A20
Host Mouse

Application Details:  CD45.1/Ly-5.1-FITC (Mouse) antibody

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Application Flow Cytometry (FACS)
Application Notes Working Dilutions: Flow Cytometry: Fluorescein (1 ug/10^6 cells), Biotin (1 ug/10^6 cells), R-phycoerythrin (0.2 ug/10^6 cells), Allophycocyanin (0.2 ug/10^6 cells), Spectral Red (0.2 ug/10^6 cells), Cyanine 5 (0.2 ug/10^6 cells), R-phycoerythrin-Cyanine 5.5 (0.2 ug/10^6 cells), R-phycoerythrin-Cyanine 7 (0.2 ug/10^6 cells), Allophycocyanin -Cyanine 5.5 (0.2 ug/10^6 cells), Allophycocyanin -Cyanine 7 (0.2 ug/10^6 cells),
Conjugate FITC
Storage The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.0. No preservatives or amine-containing buffer salts added. Store at 2-8C. The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8C. The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8C. The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8C. Do not freeze! The Allophycocyanin (APC) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8C. Do not freeze! The Spectral Red (SPRD), R-phycoerythrin-Cyanine 5.5 (R-PE-CY5.5), R-phycoerythrin-Cyanine 7 (R-PE-CY7), Allophycocyanin-Cyanine 5.5 (APC-CY5.5) and Allophycocyanin-Cyanine 7 (APC-CY7) conjugates are supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8C. Do not freeze! The Cyanine 5 (CY5) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3. Store at 2-8C. Protect conjugated forms from light. Each reagent is stable for the period shown on the bottle label if stored as directed. WARNING Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Restrictions For Research Use only

Publications:  CD45.1/Ly-5.1-FITC (Mouse) antibody

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Publications Okumura, Thomas:
"Regulation of immune function by protein tyrosine phosphatases."
In: Current opinion in immunology , Vol. 7, Issue 3, pp. 312-9, 1995/01 (PubMed)

Vallera, Taylor, Sprent et al.:
"The role of host T cell subsets in bone marrow rejection directed to isolated major histocompatibility complex class I versus class II differences of bm1 and bm12 mutant mice."
In: Transplantation , Vol. 57, Issue 2, pp. 249-56, 1994/01 (PubMed)

External Information:  CD45.1/Ly-5.1-FITC (Mouse) antibody

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Google Scholar Find more references for clone A20 on Google Scholar™
EMBL Harvester Search human, mouse or rat genome for antigen CD45.1/Ly-5.1 (Bioinformatic Harvester (EMBL))