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Product details for CD49b Mouse (Murine) antibody
CD49b Mouse (Murine) antibody
Overview
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| Antigen: | CD49b |
| Antibody Type: | Monoclonal |
| Application: | Flow Cytometry (FACS) |
| Conjugate: | PE |
| Reactivity: | Mouse (Murine) |
| Host: | Hamster |
| Quantity: | |
| Price: | 409,21 $ (Plus shipping costs and VAT, Invoice in EUR) |
| Order number: | ABIN155874 |
| Availability: | Ships within 7 to 10 days |
Additional Information: CD49b Mouse (Murine) antibody
| Antigen | CD49b |
| Immunogen | Mouse colon carcinoma cell line Colon26 |
| Reactivity | Mouse (Murine) |
| Antibody Type | Monoclonal |
| Isotype | Armenian Hamster IgG »Matching secondary antibodies |
| Description | CD49b is a 150 kD glycoprotein, also known as alpha2 integrin, VLA-2 alpha chain, Integrin alpha2 chain, and HMalpha2. It is a member of the integrin family, expressed on NK cells, a subset of splenic CD4 T cells, NK-T cells, intestinal intraepithelial and lamina propria lymphocytes, epithelial cells, and platelets. By associating with CD29 (integrin beta1 subunit), CD49b forms the VLA-2 (integrin alpha2beta1 ) complex. It plays a critical role in both adhesion and lymphocyte activation. The primary ligands for CD49b are collagen, laminin, and fibronectin. The HMalpha2 antibody has been shown to be useful for partially blocking CD49b mediated interactions with collagen. Additionally, this antibody blocks staining of splenic NK cells by the monoclonal antibody DX5. Distribution: NK cells, subset splenic CD4 T cells, NK-T cells, intestinal intraepithelial and lamina propria lymphocytes, epithelial cells, platelets Function: Adhesion, lymphocyte activation Synonyms: alpha2 integrin, VLA-2 alpha chain, Integrin alpha2 chain, HMa2 Structure: Integrin family, associates with CD29 (beta1 integrin), 150 kD |
| Clone | HMa2-HMalpha2 |
| Host | Hamster |
Application Details: CD49b Mouse (Murine) antibody
| Application | Flow Cytometry (FACS) |
| Application Notes | Additional reported applications (for the relevant formats) include: immunoprecipitation, blocking of cell adhesion, and immunohistochemistry of acetone-fixed frozen sections. The LEAF(TM) purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm sterile-filtered) is recommended for functional assays. Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is < 0.25 µg per 10 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. |
| Conjugate | PE |
| Purification | The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody. |
| Buffer | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Storage | The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. |
| Research Area | CD Antigens |
| Restrictions | For Research Use only |
Publications: CD49b Mouse (Murine) antibody
| Publications |
Inoue, Suzuki-Inoue, Dean et al.: "Integrin alpha2beta1 mediates outside-in regulation of platelet spreading on collagen through activation of Src kinases and PLCgamma2." In: The Journal of cell biology , Vol. 160, Issue 5, pp. 769-80, 2003/01 (PubMed) Sasaki, Tsuji, Jinushi et al.: "Differential regulation of VLA-2 expression on Th1 and Th2 cells: a novel marker for the classification of Th subsets." In: International immunology , Vol. 15, Issue 6, pp. 701-10, 2003/01 (PubMed) Arase, Saito, Phillips et al.: "Cutting edge: the mouse NK cell-associated antigen recognized by DX5 monoclonal antibody is CD49b (alpha 2 integrin, very late antigen-2)." In: Journal of immunology (Baltimore, Md. : 1950) , Vol. 167, Issue 3, pp. 1141-4, 2001/01 (PubMed) Miyake, Sakurai, Okumura et al.: "Identification of collagen and laminin receptor integrins on murine T lymphocytes." In: European journal of immunology , Vol. 24, Issue 9, pp. 2000-5, 1994/01 (PubMed) Noto, Kato, Okumura et al.: "Identification and functional characterization of mouse CD29 with a mAb." In: International immunology , Vol. 7, Issue 5, pp. 835-42, 1995/01 (PubMed) |
Images: CD49b Mouse (Murine) antibody
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C57BL/6 splenocytes stained with HMalpha2 PE and 145-2C11 FITC |
Product variants
External Information: CD49b Mouse (Murine) antibody
| Google Scholar | Find more references for clone HMa2-HMalpha2 on Google Scholar™ |
| EMBL Harvester | Search human, mouse or rat genome for antigen CD49b (Bioinformatic Harvester (EMBL)) |
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