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Product details for CD49b (pan-NK cells) Mouse (Murine) antibody
CD49b (pan-NK cells) Mouse (Murine) antibody
Overview
| Antigen: | CD49b (pan-NK cells) |
| Antibody Type: | Monoclonal |
| Application: | Flow Cytometry (FACS) |
| Conjugate: | Alexa Fluor 488 |
| Reactivity: | Mouse (Murine) |
| Host: | Rat |
| Quantity: | |
| Price: | 134,59 $ (Plus shipping costs and VAT, Invoice in EUR) |
| Order number: | ABIN156418 |
| Availability: | Ships within 7 to 10 days |
Additional Information: CD49b (pan-NK cells) Mouse (Murine) antibody
| Antigen | CD49b (pan-NK cells) |
| Immunogen | IL-2-propagated NK1.1 cells from C57BL/6 mice |
| Reactivity | Mouse (Murine) |
| Antibody Type | Monoclonal |
| Isotype | Rat IgM, kappa »Matching secondary antibodies |
| Description | DX5 antigen has been recently characterized as CD49b. It is a 150 kD integrin alpha chain also known as alpha2 integrin, VLA-2 alpha chain, and integrin alpha2 chain. CD49b non-covalently associates with CD29 (beta1 integrin) to form the CD49b/CD29 complex known as VLA-2, a receptor for collagen and laminin. CD49b is expressed on platelets, the majority of NK cells, NKT cells, and a small subset of CD8+ T cells (this population can be significantly increased following viral infection). DX5 is used for the identification and isolation of NK cells, and is especially useful for identifying NK cells in mice lacking the NK1.1 antigen. The DX5 clone detects cells expressing relatively high levels of CD49b and may not be useful for the detection of cells expressing low levels of CD49b. DX5 does not block NK cell killing or binding to collagen in vitro. Distribution: NK cells, subset of T cells Function: Adhesion Synonyms: alpha2 integrin, VLA-2 alpha chain, DX5, Integrin alpha2 chain Structure: Integrin alpha chain, 150 kD |
| Clone | DX5 |
| Host | Rat |
Application Details: CD49b (pan-NK cells) Mouse (Murine) antibody
| Application | Flow Cytometry (FACS) |
| Application Notes | Additional reported applications (for the relevant formats) include: complement-mediated cytotoxicity and immunohistochemical staining of formalin-fixed and paraffin-embedded tissue sections. The binding of DX5 antibody to splenic NK cells can be blocked by HMalpha2 antibody. Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is < 0.5 µg per 10 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. ** Alexa Fluor® is a registered trademark of Molecular Probes, Inc. Alexa Fluor® dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents. |
| Conjugate | Alexa Fluor 488 |
| Purification | The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 488 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 488. |
| Buffer | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Storage | The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. |
| Research Area | CD Antigens |
| Restrictions | For Research Use only |
Publications: CD49b (pan-NK cells) Mouse (Murine) antibody
| Publications |
Arase, Saito, Phillips et al.: "Cutting edge: the mouse NK cell-associated antigen recognized by DX5 monoclonal antibody is CD49b (alpha 2 integrin, very late antigen-2)." In: Journal of immunology (Baltimore, Md. : 1950) , Vol. 167, Issue 3, pp. 1141-4, 2001/01 (PubMed) Inoue, Suzuki-Inoue, Dean et al.: "Integrin alpha2beta1 mediates outside-in regulation of platelet spreading on collagen through activation of Src kinases and PLCgamma2." In: The Journal of cell biology , Vol. 160, Issue 5, pp. 769-80, 2003/01 (PubMed) Sasaki, Tsuji, Jinushi et al.: "Differential regulation of VLA-2 expression on Th1 and Th2 cells: a novel marker for the classification of Th subsets." In: International immunology , Vol. 15, Issue 6, pp. 701-10, 2003/01 (PubMed) Andoniou, van Dommelen, Voigt et al.: "Interaction between conventional dendritic cells and natural killer cells is integral to the activation of effective antiviral immunity." In: Nature immunology , Vol. 6, Issue 10, pp. 1011-9, 2005/01 (PubMed) Bourdeau, Dube, Heinonen et al.: "TC-PTP-deficient bone marrow stromal cells fail to support normal B lymphopoiesis due to abnormal secretion of interferon-{gamma}." In: Blood , Vol. 109, Issue 10, pp. 4220-8, 2007/01 (PubMed) Norian, Allen: "No intrinsic deficiencies in CD8+ T cell-mediated antitumor immunity with aging." In: Journal of immunology (Baltimore, Md. : 1950) , Vol. 173, Issue 2, pp. 835-44, 2004/01 (PubMed) Oertelt, Lian, Cheng et al.: "Anti-mitochondrial antibodies and primary biliary cirrhosis in TGF-beta receptor II dominant-negative mice." In: Journal of immunology (Baltimore, Md. : 1950) , Vol. 177, Issue 3, pp. 1655-60, 2006/01 (PubMed) Sepulveda, Cerwenka, Morgan et al.: "CD28, IL-2-independent costimulatory pathways for CD8 T lymphocyte activation." In: Journal of immunology (Baltimore, Md. : 1950) , Vol. 163, Issue 3, pp. 1133-42, 1999/01 (PubMed) |
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