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Product details for CD49b/VLA-2a-FITC (Mouse) antibody
CD49b/VLA-2a-FITC (Mouse) antibody
Overview
| Antigen: | CD49b/VLA-2a |
| Antibody Type: | Monoclonal |
| Application: | Flow Cytometry (FACS) |
| Conjugate: | FITC |
| Reactivity: | Mouse (Murine) |
| Host: | Rat |
| Quantity: | |
| Price: | 508,45 $ (Plus shipping costs and VAT, Invoice in EUR) |
| Order number: | ABIN135346 |
| Availability: | 7 to 10 days until shipment |
Additional Information: CD49b/VLA-2a-FITC (Mouse) antibody
| Antigen | CD49b/VLA-2a |
| Immunogen | RIL-2-propagated NK1.1+ cells from C57BL/6 mice 1 Fusion Partner Mouse SP2/0 myeloma cell line |
| Reactivity | Mouse (Murine) |
| Antibody Type | Monoclonal |
| Isotype | IgM »Matching secondary antibodies |
| Description | Mouse CD49b, Mr 160-kDa The monoclonal antibody DX5 reacts with CD49b, also known as very late antigen-2 (VLA-2) and 2 integrin.1 The antibody stains the majority of NK cells and a small subpopulation of T cells in all mouse strains tested (e.g., A/J, AKR, BALB/c, C3H/HeJ, C57BL/6, C57BL/10, C57BR, C58, CBA/Ca, CBA/J, DBA/1, DBA/2, SJL, SWR).2. 3 Three-color flow cytometry analysis has revealed that most cells that express NK-1.1 (NKR-P1C) also express CD49b. However, small subsets of DX5+NK-1.1- and DX5-NK-1.1+ cells can be found, especially among the CD3+ population of T lymphocytes.2 NK cells cultured in the presence of IL-2 progressively lose reactivity with DX5 as a consequence of cellular proliferation.1 Therefore, DX5 can be used to define functionally distinct subsets of murine NK cells. The monoclonal antibody DX5 has not been demonstrated to have activating or blocking activity. |
| Clone | DX5 |
| Host | Rat |
Application Details: CD49b/VLA-2a-FITC (Mouse) antibody
| Application | Flow Cytometry (FACS) |
| Application Notes | Working Dilutions: Flow Cytometry: Fluorescein (1 ug/10^6 cells), Biotin (1 uug/10^6 cells), R-phycoerythrin (0.2 ug/10^6 cells), Allophycocyanin (0.2 ug/10^6 cells), Allophycocyanin-Cyanine 7 (APC-CYTM7) (0.2 ug/10^6 cells). Since applications vary, investigators are advised to determine the optimal working dilution of the product that is appropriate for a specific need. |
| Conjugate | FITC |
| Storage | The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.0. No preservatives or amine-containing buffer salts added. Store at 2-8C. The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8C. The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8C. The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8C. Do not freeze! The Allophycocyanin (APC) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8C. Do not freeze! The Allophycocyanin-Cyanine 7 (APC-CYTM7) conjugates are supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8C. Do not freeze! The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20C. Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide-free product at -20C immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed. WARNING Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing. |
| Restrictions | For Research Use only |
Publications: CD49b/VLA-2a-FITC (Mouse) antibody
| Publications |
Arase, Saito, Phillips et al.: "Cutting edge: the mouse NK cell-associated antigen recognized by DX5 monoclonal antibody is CD49b (alpha 2 integrin, very late antigen-2)." In: Journal of immunology (Baltimore, Md. : 1950) , Vol. 167, Issue 3, pp. 1141-4, 2001/01 (PubMed) |
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