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Product details for CD49e Mouse (Murine) antibody
CD49e Mouse (Murine) antibody
Overview
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| Antigen: | CD49e |
| Antibody Type: | Monoclonal |
| Application: | Flow Cytometry (FACS), Immunoprecipitation (IP) |
| Reactivity: | Mouse (Murine), Rat |
| Host: | Hamster |
| Quantity: | |
| Price: | 338,52 $ (Plus shipping costs and VAT, Invoice in EUR) |
| Order number: | ABIN155916 |
| Availability: | Ships within 7 to 10 days |
Additional Information: CD49e Mouse (Murine) antibody
| Antigen | CD49e |
| Immunogen | Affinity-purified mouse VLA-5 protein (alpha5beta1, CD49e/CD29) |
| Reactivity | Mouse (Murine), Rat |
| Antibody Type | Monoclonal |
| Format | Purified |
| Isotype | Armenian Hamster IgG »Matching secondary antibodies |
| Description | CD49e is a 135 kD protein, also known as alpha5 integrin or VLA-5 alpha chain. It is a member of the integrin family, expressed on thymocytes, splenic B cells, activated T cells, and mast cells. CD49e associates with CD29 (integrin beta1 chain) to form the fibronectin receptor (VLA-5). CD49e plays a critical role in both adhesion and T cell costimulation. The primary ligand for CD49e/CD29 (VLA-5) is fibronectin. The HMalpha5-1 antibody has been shown to partially block CD49e mediated interactions. It has also been observed that plate-bound HMalpha5-1 antibody enhances IgE mediated degranulation and that in vivo injection of HMalpha5-1 antibody, in combination with anti-CD49d and anti-CD61 antibodies, blocks passive cutaneous anaphylaxis. Distribution: Activated T cells, mast cells Function: Adhesion, costimulation Synonyms: alpha5 integrin, VLA-5 alpha chain, Integrin alpha5 chain, Structure: Integrin family, associates with integrin beta1, 135 kD |
| Clone | HMa5-1-HMalpha5-1 |
| Host | Hamster |
Application Details: CD49e Mouse (Murine) antibody
| Application | Flow Cytometry (FACS), Immunoprecipitation (IP) |
| Application Notes | Additional reported applications (for the relevant formats) include: immunoprecipitation, in vivo inhibition of fibronectin receptor (VLA-5) functions and in vitro blocking of CD49e mediated interactions . The LEAF(TM) purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm sterile-filtered) is recommended for functional assays (ABIN155922). Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is < 0.5 µg per 10 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. |
| Purification | The antibody was purified by affinity chromatography. |
| Buffer | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Storage | The antibody solution should be stored undiluted at 4 °C. |
| Research Area | CD Antigens |
| Restrictions | For Research Use only |
Publications: CD49e Mouse (Murine) antibody
| Publications |
Hemler: "VLA proteins in the integrin family: structures, functions, and their role on leukocytes." In: Annual review of immunology , Vol. 8, pp. 365-400, 1990/01 (PubMed) Kinashi, Springer: "Adhesion molecules in hematopoietic cells." In: Blood cells , Vol. 20, Issue 1, pp. 25-44, 1995/01 (PubMed) Deregibus, Cantaluppi, Calogero et al.: "Endothelial progenitor cell derived microvesicles activate an angiogenic program in endothelial cells by a horizontal transfer of mRNA." In: Blood , Vol. 110, Issue 7, pp. 2440-8, 2007/01 (PubMed) Noto, Kato, Okumura et al.: "Identification and functional characterization of mouse CD29 with a mAb." In: International immunology , Vol. 7, Issue 5, pp. 835-42, 1995/01 (PubMed) Yasuda, Hasunuma, Adachi et al.: "Expression and function of fibronectin binding integrins on rat mast cells." In: International immunology , Vol. 7, Issue 2, pp. 251-8, 1995/01 (PubMed) |
Images: CD49e Mouse (Murine) antibody
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C57BL/6 mouse bone marrow cells stained with HMalpha5-1 biotin and detected with Sav-PE |
Product variants
External Information: CD49e Mouse (Murine) antibody
| Google Scholar | Find more references for clone HMa5-1-HMalpha5-1 on Google Scholar™ |
| EMBL Harvester | Search human, mouse or rat genome for antigen CD49e (Bioinformatic Harvester (EMBL)) |
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