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Product details for CD51/61 Human antibody
CD51/61 Human antibody
Overview
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| Antigen: | CD51/61 |
| Antibody Type: | Monoclonal |
| Application: | Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunoprecipitation (IP) |
| Reactivity: | Human, Dog (Canine), Rabbit, Cat (Feline), Chicken |
| Host: | Mouse |
| Quantity: | |
| Price: | 70,69 $ (Plus shipping costs and VAT, Invoice in EUR) |
| Order number: | ABIN158049 |
| Availability: | Ships within 7 to 10 days |
Additional Information: CD51/61 Human antibody
| Antigen | CD51/61 |
| Reactivity | Human, Dog (Canine), Rabbit, Cat (Feline), Chicken |
| Antibody Type | Monoclonal |
| Format | Purified |
| Isotype | Mouse IgG1, kappa »Matching secondary antibodies |
| Description | CD51/CD61 is an integrin complex known as alphaVbeta3. It is expressed at high levels on osteoclasts, endothelial cells, and melanoma cells and at low levels on platelets and macrophages. CD51 is a heterodimer composed of disulfide-linked 125 kD and 24 kD proteins. CD61 is also a member of the integrin family known as gpIIIa or beta3 integrin. It is a 110 kD common beta subunit of CD51/CD61 or CD41/CD61 complex. CD51/CD61, also known as the vitronectin receptor, mediates the binding of platelets to immobilized vitronectin without prior activation. Other ligands include RGD-containing proteins such as fibrinogen, fibronectin, von Willebrand factor (vWf), laminin, thrombospondin and the neural adhesion molecule L1. CD51/CD61 also mediates cell-cell adhesion via interaction with CD31. CD51/CD61 acts as an activation-independent receptor for platelet attachment and spreading on vitronectin and other RGD-containing proteins, including matrix components. The 23C6 antibody has been reported to be useful for blocking studies. Distribution: Platelets, B subset, activated T cells, monocytes/macrophages, osteoblasts, endothelial cells, melanoma Function: Adhesion, bone metabolism and apoptosis Synonyms: Vitronectin receptor, Integrin alphaV/beta3 Structure: Integrin, CD51/CD61 heterodimer, 24 kDa |
| Clone | 23C6 |
| Host | Mouse |
Application Details: CD51/61 Human antibody
| Application | Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunoprecipitation (IP) |
| Application Notes | Additional reported applications (for the relevant formats) include: immunoprecipitation, immunohistochemical staining of acetone-fixed frozen tissue sections, immunofluorescence microscopy, and blocking of cell adhesion. The LEAF(TM) purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm sterile-filtered) is recommended for functional assays (ABIN179873). Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is < 1.0 µg per 10 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. |
| Purification | The antibody was purified by affinity chromatography. |
| Buffer | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Storage | The antibody solution should be stored undiluted at 4°C. |
| Research Area | CD Antigens |
| Restrictions | For Research Use only |
Publications: CD51/61 Human antibody
| Publications |
Davies, Warwick, Totty et al.: "The osteoclast functional antigen, implicated in the regulation of bone resorption, is biochemically related to the vitronectin receptor." In: The Journal of cell biology , Vol. 109, Issue 4 Pt 1, pp. 1817-26, 1989/01 (PubMed) Nesbitt, Nesbit, Helfrich et al.: "Biochemical characterization of human osteoclast integrins. Osteoclasts express alpha v beta 3, alpha 2 beta 1, and alpha v beta 1 integrins." In: The Journal of biological chemistry , Vol. 268, Issue 22, pp. 16737-45, 1993/01 (PubMed) Deregibus, Cantaluppi, Calogero et al.: "Endothelial progenitor cell derived microvesicles activate an angiogenic program in endothelial cells by a horizontal transfer of mRNA." In: Blood , Vol. 110, Issue 7, pp. 2440-8, 2007/01 (PubMed) Horton, Taylor, Arnett et al.: "Arg-Gly-Asp (RGD) peptides and the anti-vitronectin receptor antibody 23C6 inhibit dentine resorption and cell spreading by osteoclasts." In: Experimental cell research , Vol. 195, Issue 2, pp. 368-75, 1991/01 (PubMed) Takahashi, Sekine, Nakatake: "Influence of monoclonal antiplatelet glycoprotein antibodies on in vitro human megakaryocyte colony formation and proplatelet formation." In: Blood , Vol. 93, Issue 6, pp. 1951-8, 1999/01 (PubMed) |
Images: CD51/61 Human antibody
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Human melanoma cell line M21 stained with purified 23C6, followed by anti-mouse IgGs FITC |
Product variants
External Information: CD51/61 Human antibody
| Google Scholar | Find more references for clone 23C6 on Google Scholar™ |
| EMBL Harvester | Search human, mouse or rat genome for antigen CD51/61 (Bioinformatic Harvester (EMBL)) |
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