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Product details for CD52 Human antibody
CD52 Human antibody
Overview
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| Antigen: | CD52 |
| Antibody Type: | Monoclonal |
| Application: | Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) |
| Reactivity: | Human |
| Host: | Mouse |
| Quantity: | |
| Price: | 149,54 $ (Plus shipping costs and VAT, Invoice in EUR) |
| Order number: | ABIN158895 |
| Availability: | Ships within 7 to 10 days |
Additional Information: CD52 Human antibody
| Antigen | CD52 |
| Immunogen | Human tonsil |
| Reactivity | Human |
| Antibody Type | Monoclonal |
| Format | Purified |
| Isotype | Mouse IgG2b, kappa »Matching secondary antibodies |
| Description | CD52, also known as Cambridge pathology antigen 1 (CAMPATH-1), is a 25-29 kD glycoprotein containing a large N-linked carbohydrate moiety. The actual molecule of CD52 is only 8-9 kD. It is expressed in the male reproductive tract and on virtually all lymphocytes (T and B cells), as well as macrophages/monocytes, eosinophils, and red cells. CD52 is thought to play a role in carrying and orienting carbohydrates. CD52 is a potent target for complement-mediated lysis and antibody-mediated cellular cytotoxicity and has been used as a depletion target for chronic lymphocytic leukemia (CLL)/lymphoma and immunosuppression. The HI186 antibody is useful for flow cytometry and immunohistochemistry. Distribution: Male reproductive tract (epididymis, seminal vesicle), T cells, B cells, macrophage/monocyte, eosinophil, erythrocytes, malignant lymphocytes Function: Thought to play a role in carrying and orienting carbohydrates. Target for complement-mediated lysis and antibody-dependent cellular cytotoxicity, used as a depletion target for chronic lymphocytic leukemia/lymphoma therapy and immunosuppression. Synonyms: Cambridge pathology antigen 1 (CAMPATH-1), epididymal secretory protein 1 Structure: Unusually small glycopeptide containing N-linked carbohydrate moiety, GPI-linked membrane protein, approximate molecular weight 7 kD |
| Clone | HI186 |
| Host | Mouse |
Application Details: CD52 Human antibody
| Application | Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) |
| Application Notes | Additional reported applications (for the relevant formats) include: immunohistochemical staining of formalin-fixed paraffin-embedded tissue sections. Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is < 1.0 µg per 10 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. |
| Purification | The antibody was purified by affinity chromatography. |
| Buffer | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Storage | The antibody solution should be stored undiluted at 4°C. |
| Research Area | CD Antigens |
| Restrictions | For Research Use only |
Publications: CD52 Human antibody
| Publications |
Kirchhoff, Krull, Pera et al.: "A major mRNA of the human epididymal principal cells, HE5, encodes the leucocyte differentiation CDw52 antigen peptide backbone." In: Molecular reproduction and development , Vol. 34, Issue 1, pp. 8-15, 1993/01 (PubMed) Xia, Tone, Packman et al.: "Characterization of the CAMPATH-1 (CDw52) antigen: biochemical analysis and cDNA cloning reveal an unusually small peptide backbone." In: European journal of immunology , Vol. 21, Issue 7, pp. 1677-84, 1991/01 (PubMed) Xia, Hale, Lifely et al.: "Structure of the CAMPATH-1 antigen, a glycosylphosphatidylinositol-anchored glycoprotein which is an exceptionally good target for complement lysis." In: The Biochemical journal , Vol. 293 ( Pt 3), pp. 633-40, 1993/01 (PubMed) |
Images: CD52 Human antibody
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Human peripheral blood lymphocytes stained with purified HI186, followed by anti-mouse IgGs FITC |
Product variants
External Information: CD52 Human antibody
| Google Scholar | Find more references for clone HI186 on Google Scholar™ |
| EMBL Harvester | Search human, mouse or rat genome for antigen CD52 (Bioinformatic Harvester (EMBL)) |
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