CD8 Rat antibody

Overview

Antigen:	CD8
Antibody Type:	Monoclonal
Application:	Flow Cytometry (FACS)
Conjugate:	FITC
Reactivity:	Rat
Host:	Mouse
Quantity:	100 µg (Product variants)
Price:	151,00 € (Plus shipping costs and VAT)
Order number:	ABIN157369
Availability:	Ships within 7 to 10 days

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Additional Information: CD8 Rat antibody

Antigen	CD8
Immunogen	High molecular weight glycoproteins from rat thymocytes
Reactivity	Rat
Antibody Type	Monoclonal
Isotype	Mouse IgG1 Mouse IgG1, kappa »Matching secondary antibodies
Description	CD8a is a 32 kD glycoprotein also known as T8, Lyt2, Ly-2, and CD8alpha. CD8a is a member of the immunoglobulin superfamily expressed on most thymocytes, subset of mature T cells, most NK cells, macrophages, and some activated CD4 T cells (not resting). CD8a forms heterodimers with the CD8beta chain (CD8b) on the surface of most thymocytes, while mature peripheral T lymphocytes express almost exclusively the CD8 alphabeta heterodimer. Intestinal intraepithelial lymphocytes express CD8a without CD8b. CD8 is an antigen co-receptor on T cells that interacts with MHC class I on antigen-presenting cells or epithelial cells. CD8 participates in T cell activation through its association with the T cell receptor complex and protein tyrosine kinase lck (p56lck). The OX-8 antibody has been reported to partially block T cell responses, including mixed lymphocyte reactions and cytotoxic T cell responses, and to induce macrophage activation. Distribution: Thymocytes, NK cells, most CD8 cells, intestinal intraepithelial lymphocytes, activated CD4 cells, macrophages Function: T cell activation, co-receptor for T cell activation Synonyms: CD8a, T8, Leu-8, Ly-2, Lyt2 Structure: Ig superfamily, approximately 32 kD
Clone	OX-8 -OX-8
Host	Mouse

Application Details: CD8 Rat antibody

Application	Flow Cytometry (FACS)
Application Notes	Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections, immunoprecipitation, in vivo and in vitro blocking of T cell responses, macrophage stimulation, and Western blotting. Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is < 0.5 µg per 10 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Conjugate	FITC
Purification	The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC.
Buffer	Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.5% BSA (origin USA).
Storage	The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Research Area	CD Antigens
Restrictions	For Research Use only

Publications: CD8 Rat antibody

Publications

Johnson, Gagnon, Barclay et al.:
"Purification, chain separation and sequence of the MRC OX-8 antigen, a marker of rat cytotoxic T lymphocytes."
In: The EMBO journal , Vol. 4, Issue 10, pp. 2539-45, 1985/01 (PubMed)

Thomas, Green:
"Molecular nature of the W3/25 and MRC OX-8 marker antigens for rat T lymphocytes: comparisons with mouse and human antigens."
In: European journal of immunology , Vol. 13, Issue 10, pp. 855-8, 1984/01 (PubMed)

Barclay:
"The localization of populations of lymphocytes defined by monoclonal antibodies in rat lymphoid tissues."
In: Immunology , Vol. 42, Issue 4, pp. 593-600, 1981/01 (PubMed)

Hirji, Lin, Befus:
"A novel CD8 molecule expressed by alveolar and peritoneal macrophages stimulates nitric oxide production."
In: Journal of immunology (Baltimore, Md. : 1950) , Vol. 158, Issue 4, pp. 1833-40, 1997/01 (PubMed)

Mason, Arthur, Dallman et al.:
"Functions of rat T-lymphocyte subsets isolated by means of monoclonal antibodies."
In: Immunological reviews , Vol. 74, pp. 57-82, 1983/01 (PubMed)

Mitnacht, Bischof, Torres-Nagel et al.:
"Opposite CD4/CD8 lineage decisions of CD4+8+ mouse and rat thymocytes to equivalent triggering signals: correlation with thymic expression of a truncated CD8 alpha chain in mice but not rats."
In: Journal of immunology (Baltimore, Md. : 1950) , Vol. 160, Issue 2, pp. 700-7, 1998/01 (PubMed)

Torres-Nagel, Kraus, Brown et al.:
"Differential thymus dependence of rat CD8 isoform expression."
In: European journal of immunology , Vol. 22, Issue 11, pp. 2841-8, 1992/01 (PubMed)

Wallgren, Karlsson-Parra, Korsgren:
"The main infiltrating cell in xenograft rejection is a CD4+ macrophage and not a T lymphocyte."
In: Transplantation , Vol. 60, Issue 6, pp. 594-601, 1995/01 (PubMed)

Product variants

CD8 Rat antibody (Format: Purified, Quantity: 100 µg)

CD8 Rat antibody (Quantity: 50 µg, Conjugate: PE)

External Information: CD8 Rat antibody

Google Scholar	Find more references for clone OX-8 -OX-8 on Google Scholar™
EMBL Harvester	Search human, mouse or rat genome for antigen CD8 (Bioinformatic Harvester (EMBL))