Product details for  CD8-Biotin (Feline) antibody
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CD8-Biotin (Feline) antibody

Overview

Antigen: CD8
Antibody Type: Monoclonal
Conjugate: Biotin
Reactivity: Cat (Feline)
Host: Mouse
Quantity: 0,5 mg
Price: 312,00 €   (Plus shipping costs and VAT)
Order number: ABIN135498
Availability: 7 to 10 days until shipment
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Additional Information:  CD8-Biotin (Feline) antibody

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Antigen CD8
Reactivity Cat (Feline)
Antibody Type Monoclonal
Isotype IgG1 »Matching secondary antibodies
Description Feline CD8, Mr 70 kDa (38/31 kDa under reducing conditions). Feline CD8, a member of the immunoglobulin superfamily of cell surface receptors, is a type II transmembrane glycoprotein that is expressed as a heterodimer on the suppressor/cytotoxic subpopulation of peripheral T lymphocytes. It is present on approximately 63% of thymocytes, 9% of splenocytes, 20% of lymph node cells, and 15% of peripheral blood lymphocytes.1,2 CD8 functions as a co-receptor with MHC Class I-restricted T cell receptors in antigen recognition.
Clone FCD8
Host Mouse

Application Details:  CD8-Biotin (Feline) antibody

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Application Notes Working Dilutions: Flow Cytometry: Fluorescein (1 ug/10^6 cells), Biotin (1 ug/10^6 cells), R-phycoerythrin (0.2 ug/10^6 cells). Since applications vary, you should determine the optimum working dilution of the product that is appropriate for your specific need.
Conjugate Biotin
Storage The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.0. No preservatives or amine-containing buffer salts added. Store at 2-8C. The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8C. The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8C. The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8C. Do not freeze! Protect conjugated forms from light. Each reagent is stable for the period shown on the bottle label if stored as directed. WARNING Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Research Area CD Antigens, Surface Receptors of Immune Cells
Restrictions For Research Use only

Publications:  CD8-Biotin (Feline) antibody

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Publications Dean, Quackenbush, Ackley et al.:
"Flow cytometric analysis of T-lymphocyte subsets in cats."
In: Veterinary immunology and immunopathology , Vol. 28, Issue 3-4, pp. 327-35, 1991/01 (PubMed)

Klotz, Cooper:
"A feline thymocyte antigen defined by a monoclonal antibody (FT2) identifies a subpopulation of non-helper cells capable of specific cytotoxicity."
In: Journal of immunology (Baltimore, Md. : 1950) , Vol. 136, Issue 7, pp. 2510-4, 1986/01 (PubMed)

External Information:  CD8-Biotin (Feline) antibody

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Google Scholar Find more references for clone fCD8 on Google Scholar™
EMBL Harvester Search human, mouse or rat genome for antigen CD8 (Bioinformatic Harvester (EMBL))