Product details for  CD8a Mouse (Murine) antibody
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CD8a Mouse (Murine) antibody

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Image for CD8a Mouse (Murine) antibody
Antigen: CD8a
Antibody Type: Monoclonal
Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunhistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Depletion, Blocking Antibody (BLOCK)
Conjugate: LEAF
Reactivity: Mouse (Murine)
Host: Rat
Quantity: 500 µg (Product variants)
Price: 226,00 €   (Plus shipping costs and VAT)
Order number: ABIN155451
Availability: Ships within 7 to 10 days
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Additional Information:  CD8a Mouse (Murine) antibody

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Antigen CD8a
Immunogen Mouse thymus or spleen
Reactivity Mouse (Murine)
Antibody Type Monoclonal
Format Sterile Low-Endotoxin, Azide-Free formulation (LEAF)
Isotype Rat IgG2a, kappa »Matching secondary antibodies
Description CD8, also known as Lyt-2, Ly-2, or T8, consists of disulfide-linked alpha and beta chains that form the alpha(CD8a)/beta(CD8b) heterodimer and alpha/alpha homodimer. CD8a is a 34kD protein that belongs to the immunoglobulin family. The CD8 alpha/beta heterodimer is expressed on the surface of most thymocytes and a subset of mature TCR alpha/beta T cells. CD8 expression on mature T cells is non-overlapping with CD4. The CD8 alpha/alpha homodimer is expressed on a subset of gamma/delta TCR-bearing T cells, NK cells, intestinal intraepithelial lymphocytes, and lymphoid dendritic cells. CD8 is an antigen co-receptor on T cells that interacts with MHC class I on antigen-presenting cells or epithelial cells. CD8 promotes T cell activation through its association with the TCR complex and protein tyrosine kinase lck. The 53-6.7 antibody has been reported to block antigen presentation via MHC class I and inhibit T cell responses to IL-2. This antibody has also been used for depletion of CD8a cells. Distribution: Most thymocytes, T cell subset, some NK cells Function: Co-receptor for TCR Synonyms: T8, Lyt2, Ly-2 Structure: Ig superfamily, CD8alpha chain, 34 kD
Clone 53-6-7
Host Rat

Application Details:  CD8a Mouse (Murine) antibody

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Application Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunhistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Depletion, Blocking Antibody (BLOCK)
Application Notes Additional reported applications (for the relevant formats) include: immunoprecipitation, in vivo and in vitro cell depletion, inhibition of CD8 T cell proliferation, blocking of cytotoxicity, and immunohistochemical staining of acetone-fixed frozen sections, zinc-fixed paraffin-embedded sections and 10% formalin-fixed paraffin-embedded sections. Special Note: The antibody works well on acetone-fixed frozen sections as well as Zinc-fixed paraffin-embedded sections. It sometime works on formalin-fixed paraffin-embedded tissue sections but inconsistent results have been reported. The 53-6.7 antibody competes with 5H10-1 antibody (ABIN155467) for binding to thymocytes. The LEAF(TM) purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm sterile-filtered) is recommended for functional assays (ABIN155451). Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is < 0.25 µg per 10 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Conjugate LEAF
Purification The antibody was purified by affinity chromatography.
Buffer Phosphate-buffered solution, pH 7.2, containing no preservative. 0.2 µm filter sterilized. Endotoxin level is < 0.1 EU/µg of the protein (< 0.01 ng/µg of the protein) as determined by the LAL test.
Storage The antibody solution should be stored undiluted at 4 °C. This LEAF(TM) solution contains no preservative, handle under aseptic conditions.
Research Area CD Antigens
Restrictions For Research Use only

Publications:  CD8a Mouse (Murine) antibody

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Publications Ellmeier, Sawada, Littman:
"The regulation of CD4 and CD8 coreceptor gene expression during T cell development."
In: Annual review of immunology , Vol. 17, pp. 523-54, 1999/01 (PubMed)

Zamoyska:
"The CD8 coreceptor revisited: one chain good, two chains better."
In: Immunity , Vol. 1, Issue 4, pp. 243-6, 1995/01 (PubMed)

Bouwer, Alberti-Segui, Montfort et al.:
"Directed antigen delivery as a vaccine strategy for an intracellular bacterial pathogen."
In: Proceedings of the National Academy of Sciences of the United States of America , Vol. 103, Issue 13, pp. 5102-7, 2006/01 (PubMed)

Fan, Kouda, Nakamura et al.:
"Effects of dietary restriction on spontaneous dermatitis in NC/Nga mice."
In: Experimental biology and medicine (Maywood, N.J.) , Vol. 226, Issue 11, pp. 1045-50, 2001/01 (PubMed)

Hata, Sakaguchi, Yoshitomi et al.:
"Distinct contribution of IL-6, TNF-alpha, IL-1, and IL-10 to T cell-mediated spontaneous autoimmune arthritis in mice."
In: The Journal of clinical investigation , Vol. 114, Issue 4, pp. 582-8, 2004/01 (PubMed)

Kamimura, Sawa, Sato et al.:
"IL-2 in vivo activities and antitumor efficacy enhanced by an anti-IL-2 mAb."
In: Journal of immunology (Baltimore, Md. : 1950) , Vol. 177, Issue 1, pp. 306-14, 2006/01 (PubMed)

Ko, Ko, Chang et al.:
"alpha-Galactosylceramide can act as a nasal vaccine adjuvant inducing protective immune responses against viral infection and tumor."
In: Journal of immunology (Baltimore, Md. : 1950) , Vol. 175, Issue 5, pp. 3309-17, 2005/01 (PubMed)

Ledbetter, Herzenberg:
"Xenogeneic monoclonal antibodies to mouse lymphoid differentiation antigens."
In: Immunological reviews , Vol. 47, pp. 63-90, 1980/01 (PubMed)

Ledbetter, Seaman, Tsu et al.:
"Lyt-2 and lyt-3 antigens are on two different polypeptide subunits linked by disulfide bonds. Relationship of subunits to T cell cytolytic activity."
In: The Journal of experimental medicine , Vol. 153, Issue 6, pp. 1503-16, 1981/01 (PubMed)

Shih, Racz, Allen:
"Differential MHC class II presentation of a pathogenic autoantigen during health and disease."
In: Journal of immunology (Baltimore, Md. : 1950) , Vol. 176, Issue 6, pp. 3438-48, 2006/01 (PubMed)

Takahashi, Nakata, Tanaka et al.:
"CD4 and CD8 regulate interleukin 2 responses of T cells."
In: Proceedings of the National Academy of Sciences of the United States of America , Vol. 89, Issue 12, pp. 5557-61, 1992/01 (PubMed)

Images:  CD8a Mouse (Murine) antibody

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C57BL/6 mouse splenocytes stained with LEAF(TM) purified 53-6.7, followed by anti-rat IgGs-FITC

Image for CD8a Mouse (Murine) antibody (1)

External Information:  CD8a Mouse (Murine) antibody

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Google Scholar Find more references for clone 53-6-7 on Google Scholar™
EMBL Harvester Search human, mouse or rat genome for antigen CD8a (Bioinformatic Harvester (EMBL))